摘要
目的研究癫痫大鼠ALK5对ALK1受体的作用,探讨可能的干预癫痫发作的新靶点。方法红藻氨酸(kainic acid,KA)侧脑室注入SD大鼠制备癫痫模型,随机分为KA模型对照组(A组)、ALK5抑制剂(SB431542)腹腔注射3 d组(B组)、ALK5抑制剂腹腔注射7 d组(C组),另设假手术组为空白对照组(NC组),每组各10只。NC组、A组、B组分别于3 d,C组于7 d取海马,检测海马组织中ALK1和其下游分子p-Smad1的mRNA及其蛋白的表达。结果与正常对照组相比,KA模型对照组大鼠海马区ALK1和p-Smad1的mRNA及蛋白表达均升高,差异有统计学意义(P<0.05);与KA模型组相对比,ALK5抑制剂腹腔注射组(B组和C组)ALK1和pSmad1的mRNA及蛋白表达均下降(P<0.05)。结论 ALK5抑制剂腹腔注射后导致KA诱导的SD癫痫大鼠ALK1受体和p-Smad1表达下调。
Abstract： Objective To observe the expression of the mRNA and the protein level of the ALK1 receptor and it＇ s downstream molecules p-Smadl and study the function of ALK5 receptor to ALK1 receptor after injection of ALK5 inhibitor （SB431542） , in order to explore new targets for possible intervention of epileptic seizures. Methods Male Sprague-Dawley rats were taken for the epileptic model kindled by Kainie acid （KA） and were divided into three groups randomly as KA control group（A） ,ALK5 inhibitor 3 clays group （B） ,ALK5 inhibitor 7 days group （C）. Besides, take sham rats for the normal control group. The normal control group and KA group were injected with saline in abdominal cavity for three clays ;The ALK5 inhibitor 3 clays group rats intraperitoneally infused with ALK5 inhibitor for three days ,and the ALK5 inhibitor 7 clays group rats for seven days. The expression of the mRNA and the protein of the ALK1 receptor and p-Smadl in the rats hippoeampus neurons were examined by RT-PCR and immunofluorescenee histochemistry. Results The expression of he ALK1 receptor and p-Smadl in KA group increased （ P 〈 0.05 ） compared with normal control group in the real time quantitative PCR and immunohistoehemistry analysis,in which ALK5 inhibitor 3 days group and ALK5 inhibitor 7 clays group dereased （ P 〈 0. 05 ） compared with KA group. Conclusion After ALK5 inhibitor was injected into the abdominal cavity,the expression of ALK1 receptor and p-Smad1 decreased.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2014年第11期977-981,共5页
Journal of Apoplexy and Nervous Diseases
基金
国家自然科学基金项目(No.81160167)
