摘要
目的观察孕酮对大脑中动脉栓塞(MCAO)大鼠脑组织TASK3蛋白表达的影响,探讨孕酮对脑缺血再灌注损伤大鼠的脑保护作用及机制。方法雄性SD大鼠随机分为正常对照组、假手术组、缺血再灌注组(I/R组)和孕酮+I/R组,后3组按缺血再灌注后不同时间随机分为0 h、6 h、12 h、24 h、48 h、72 h、96 h和120 h 8个亚组。线栓法建立大鼠右侧MCAO模型,缺血2 h再灌注24 h进行脑损伤评估,检测各组大鼠死亡率、神经功能缺陷评分以及2,3,5-氯化三苯基四氮唑(TTC)染色测定脑梗死体积;采用免疫印迹法检测各组大鼠脑组织缺血2 h再灌注后不同时间点TASK3蛋白的表达。结果孕酮+I/R组大鼠死亡率(18.18%)明显低于I/R组(40.66%),该组动物脑梗死体积(21.85%±3.53%)也显著低于I/R组(37.21%±3.50%)(P<0.01),且该组神经功能缺陷评分(2.00±0.74)显著低于I/R组(2.67±0.49)(P<0.05)。与正常对照组相比,假手术组各时间点脑组织TASK3蛋白的表达无明显变化,而I/R组各时间点脑组织TASK3蛋白的表达均显著降低(P<0.05);与I/R组相比,孕酮+I/R组各时间点脑组织TASK3蛋白的表达显著升高(P<0.05)。结论孕酮对脑缺血再灌注损伤的大鼠具有脑保护作用,其机制可能与上调TASK3通道蛋白的表达有关。
Objective To investigate the effect of progesterone (PROG) on the expression of Two-pore domain K channel 3 (TASK3) after cerebral ischemia-reperfusion injury in rats. Methods Male Sprague-Dawley rats were divided into four groups randomly : normal control group, sham-operated group, ischemia-reperfusion group ( I/R group) and PROG + I/R group. Sham-operated group,I/R group and PROG + I/R group were divided into 0 h,6 h,12 h,24 h,48 h,72 h,96 h and 120 h eight subtypes according to different reperfusion time. The model of MCAO was established by the line-embolism method. The mortality rate, the nervous functional defect degree and the volumes of cerebral infarction were evaluated when rats were suffered 2 h/24 h ischemia-reperfusion stress. The expression of TASK3 protein in right brain was measured by Western blotting assay at reperfusion time of 0 h,6 h,12 h,24 h,48 h,72 h,96 h and 120 h after 2 h ischemia. Results The mortality rate in PROG + I/R rats ( 18.18% ) were obviously lower than that of I/R group(40.66% ). The nervous defect scores in PROG + I/R rats (2.00 ± 0.74) were significant lower than that of I/R group ( 2.67 ± 0.49 ) ( P 〈 0.05 ). The volumes of cerebral infarction in PROG + I/R group (21.85% ± 3.53% ) were significant lower than that of I/R group (37.21% ± 3.50% ) ( P 〈 0.01 ). No significant difference of the protein expression of TASK3 in brain tissues at all time points between normal control group and sham-operated group was detected. However, the protein expression of TASK3 in brain tissue at all time points significant decreased in I/R group as compared with normal control group ( P 〈 0.05 ). Compared with I/R group, the protein expression of TASK3 in brain tissue at all time points dramatically increased in PROG + I/R group ( P 〈 0.05 ). Conclusion Progesterone exerts the neuroprotective effect on focal cerebral ischemia/ reperfusion injury in rats,the protective mechanism might be associated with up-regulating the expression of TASK3 protein in brain.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2014年第11期991-994,共4页
Journal of Apoplexy and Nervous Diseases
基金
国家自然科学基金资助项目(No.81100912
81371346)
新乡医学院优秀硕士资助课题
关键词
大鼠
孕酮
脑缺血
再灌注损伤
TASK3
Rat
Progesterone
Cerebral ischemia
Reperfusion injury
Two-pore domain K channel 3