砷对大鼠生精细胞半胱氨酸天冬氨酸酶-3表达及血清中黄体生成素浓度的影响

Effects of arsenic on casapse-3 in spermatogenic cells and LH in serum of rats

目的研究亚慢性砷中毒对大鼠睾丸生精细胞半胱氨酸天冬氨酸酶-3(caspase-3)表达及血清黄体生成素(LH)的影响。方法将40只健康成年清洁级SD雄性大鼠随机分为4组,分别为对照组(蒸馏水)组和0.4、2、10 mg/kg亚砷酸钠染毒组,每组10只,采用自由饮水方式进行染毒,连续染毒14周。测定大鼠血清LH浓度及生精细胞caspase-3阳性率。结果与对照组比较,各剂量亚砷酸钠染毒组大鼠生精细胞caspase-3阳性率均较高,2、10 mg/kg亚砷酸钠染毒组大鼠血清LH浓度均较低,差异有统计学意义(P<0.01,P<0.05);且随着亚砷酸钠暴露剂量的升高,大鼠生精细胞caspase-3阳性率呈上升趋势,血清LH浓度呈下降趋势。结论砷暴露导致的生精细胞caspase-3表达增加可能是其雄性生殖毒性效应的机制之一。

Objective To investigate the effects of subchronic arsenic exposure on caspase-3 expression in spermatogenic cells and LH in serum of rats. Methods Forty SD rats were randomly divided into four groups,10 in each and treated with sodium arsenite through drinking water at the doses of 0, 0.4, 2 and 10 mg/kg for 14 weeks. Morphological changes of testis were examined by histological technique-HE staining;LH in serum was determined by ELISA;The expression of caspase-3 was assayed by immunohistochemical staining. Results Compared with the control group,LH in serum in 2 and 10 mg/kg groups decreased significantly（P 〈0.05）;The positive expression rates of caspase-3 in spermatogenic cells in 0.4, 2 and 10 mg/kg groups increased significantly（P〈0.01）. Conclusion Up-regulation the expression of caspase-3 may be one of the mechanisms of male germ cell toxicity induced by sodium arsenite.