高尔基体蛋白73单独及联合甲胎蛋白检测在肝细胞癌诊断中的价值

Diagnostic value of Golgi protein 73 alone and in combination with alpha fetoprotein in hepatocellular carcinoma

目的探讨血清高尔基体蛋白(Golgi protein,GP)73单独及联合甲胎蛋白(AFP)检测在肝细胞癌(hepatocellular carcinoma,HCC)诊断中的价值。方法选择HCC、肝炎、非HCC的其他肿瘤患者和健康人群,分别以酶联免疫吸附试验检测GP73水平,电化学发光法检测AFP水平。结果 HCC组GP73水平为(231.2±92.3)ng/ml,明显高于肝炎组、其他肿瘤组和健康对照组,差异有统计学意义(P<0.005)。GP73在81.08 ng/ml处获得最大约登指数,此时诊断HCC的灵敏度和特异度分别为86.2%和87.8%,高于AFP诊断的价值。GP73联合AFP诊断HCC,可使诊断灵敏度进一步提高至92.3%。结论 GP73作为一种新型HCC血清标记物在HCC诊断中有较高的灵敏度和特异度,且优于AFP。GP73联合AFP检测可进一步提高HCC诊断的灵敏度。

Objective To investigate the value of serum Golgi protein 73（GP73） alone and in combination with alpha fetoprotein（AFP） in detecting hepatocellular carcinoma（HCC）. Methods The levels of GP73 and AFP in the peripheral blood of patients with HCC, hepatitis, other tumors without HCC and controls were detected by ELISA and electrochemiluminescence immunoassay（ECLIA）, respectively. Results The average level of GP73 in HCC patients was 231.2±92.3 ng/ml, markedly higher than the levels of hepatitis group, non-HCC tumor group and the control group, respectively, and the differences between the levels were significant（P〈0.005）. The maximal Youden＇s index was obtained when GP73 was 81.08 ng/ml, and the corresponding sensitivity and specificity of GP73 level in the diagnosis of HCC were 86.2% and 87.8%, higher than those of AFP level. The combined detection of GP73 and AFP levels in the diagnosis of HCC made the sensitivity further increase to 92.3%. Conclusions GP73, as a novel serum marker, has a higher sensitivity and specificity in the diagnosis of HCC, and is superior to AFP. Combined detection of GP73 and AFP can further improve the sensitivity in the diagnosis of HCC.