紫地榆牙膏对牙釉质脱矿和再矿化作用的研究

Effects of Geranium Strictipes Toothpaste on Enamel Demineralization and Remineralization

目的评价紫地榆不同溶剂提取物牙膏抑制釉质脱矿和促进再矿化作用的影响.方法检测紫地榆不同溶剂提取物牙膏处理离体牙前后乳酸盐缓冲液中磷离子浓度的变化,测定出磷溶出率,评价紫地榆不同溶剂提取物牙膏抑制釉质脱矿作用;此外,制作人工龋釉质块模型,取洛氏硬度值无统计学差异的25例脱矿釉质块,随机分为A、B、C、D、E 5组(P>0.05),分别进行体外再矿化,检测经紫地榆不同溶剂提取物牙膏处理前后釉质表面显微硬度值,从而评价紫地榆不同溶剂提取物牙膏对釉质再矿化的作用.结果乙酸乙酯组、正丁醇组、水层组的釉质溶解度降低率与空白牙膏组比较有统计学意义(P<0.01),但3组的作用无统计学意义(P>0.05);而对于再矿化前后牙釉质表层显微硬度值,除乙酸乙酯牙膏组和空白牙膏组变化差异无统计学意义(P>0.05),正丁醇牙膏组和水层牙膏组再矿化前后牙釉质表层显微硬度值变化有统计学意义(P<0.01),但均低于NaF牙膏组(P<0.01).结论紫地榆3种不同溶剂提取物都具有抑制釉质脱矿的作用,且3种提取物的作用无统计学意义.正丁醇取物牙膏组和水层牙膏组均能促进人工脱矿牛切牙再矿化,但效果弱于NaF牙膏组(20g/L).

Objective To evaluate the effect of different solvent extracts of Geranium Strictipes toothpaste in inhibiting enamel demineralization and remineralization of demineralized enamel. Methods We detected the phosphorus ion concentration of lactate buffer and calculate the ratio of soluble phosphorus in free teeth before and after treated by toothpaste with different solvent extracts of Geranium Strictipes, then evaluated the effects of Geranium Strictipes extracts with different solvents toothpaste in inhibiting enamel demineralization. In addition,we made artificial carious enamel block model, and selected 25 cases with similar Rockwell hardness value and randomly divided them into A,B,C,D,E 5 groups, then given them remineralization in vitro, then evaluated the effect of different solvent extracts of Geranium Strictipes toothpaste on enamel remineralization through detecting the enamel surface microhardness before and after treatment. Results Compared with blank group of toothpaste,the decrease rate of enamel solubility in ethyl acetate group, n-butyl alcohol group,water group were significantly different（P0.001）,but there were no significant differences between the 3 groups（P0.05）.The enamel surface microhardness had no significant difference between before and after remineralization in Ethyl acetate toothpaste group and the blank toothpaste group（P0.05）,the enamel surface microhardness had significant difference in n-butanol toothpaste group and water toothpaste group（P0.01）,but all were lower than those in NaF group（P0.01）.Conclusion The 3 different solvent extracts of Geranium Strictipes all have the effect of inhibiting enamel demineralization,and there is no significant differences among the 3 kinds of extracts. N-butanol toothpaste group and water toothpaste group could promote the artificial demineralization of bovine incisor tooth remineralization,but the effect is weaker than that of NaF toothpaste group（20 g/L）.