乳腺癌细胞MDA-MB-435s中侧群细胞的分选及其与非侧群细胞的生物学特点比较

Separation of Side Population Cells from Breast Cancer Cell MDA-MB-435s and Comparing the Biological Characteristics with Non-side Population Cells

目的分离乳腺癌细胞MDA-MB-435s中的侧群细胞(sidepopulation cells,SP),并比较侧群细胞和非侧群细胞(non-side population cells,NSP)的基本生物学特点.方法经Hoechst33342染色后,利用流式细胞技术分选乳腺癌细胞MDA-MB-435s中SP和NSP 2个细胞亚群,比较2群细胞的形态、增殖和周期等特点.结果分选得到的SP细胞约占5.2%,其增殖速度较NSP细胞快,且细胞形态较粗大.NSP细胞的G1期所占比例为73.63%,高于SP细胞的G1期所占比例为54.16%.结论 SP和NSP细胞的形态有差异,SP细胞的增殖速度快于NSP细胞,NSP细胞的周期阻滞在G1期,提示SP细胞可能在乳腺癌细胞的生长过程中具有重要的作用.

Objective To isolate the side population cells（side population cells, SP） from breast cancer cells MDA-MB-435 s, and compare the characteristics of the basic biology with non-side population cells（non-side population cells,NSP） ＇s.Methods After Hoechst33342 staining, flow cytometry was used for sorting both SP and NSP cells from breast cancer cells MDA-MB-435 s. The morphology,proliferation and cycle characteristics were compared between SP and NSP cells.Results SP cells approximately accounted for 5.2%after sorting,the proliferation rate of SP cells was higher than NSP cells,and the morphology of SP cells were coarser. NSP proportion of cells in G1 phase was 73.63%,which was higher than the proportion of SP cells in G1phase（54.16%）.Conclusion Morphological differences were found between NSP and SP cells,the cell proliferation rate of SP cells is higher than NSP cells. NSP cell cycle arrest in the G1 phase,suggesting that SP cells may have an important role in the growth of breast cancer cells.