甲状腺乳头状癌患者肿瘤组织和血清中miR-21的表达及其意义

The expression of miR-21 in carcinoma tissues and serum of patients with papillary thyroid carcinoma

目的检测miR-21在甲状腺乳头状癌(PTC)患者肿瘤组织和血清中的表达情况,探讨其临床意义。方法采用实时荧光定量聚合酶链反应(Real-time RT-PCR)检测46例PTC患者肿瘤组织和血清中miR-21表达情况,并与35例结节性甲状腺肿患者和18例正常人进行对比分析。结果 PTC患者肿瘤组织和血清miR-21表达相对水平为2.74±1.53和0.21±0.08,明显高于结节性甲状腺肿患者(0.92±0.81和0.08±0.05)和正常人(0.86±0.39和0.07±0.03)(P<0.05)。PTC患者肿瘤组织和血清中miR-21表达具有一致性。PTC患者肿瘤组织和血清中miR-21的表达与腺外侵润、远处转移、淋巴结转移和不同TNM分期有关(P<0.05)。结论 miR-21在PTC患者肿瘤组织和血清中的表达明显升高,且其表达水平与PTC临床病理特征相关,可能是新的PTC早期检测指标。临床上对于PTC肿瘤组织和血清中miR-21的检测可能用于PTC早期临床诊断。

Objective To investigates the expression of miR-21 in carcinoma tissues and serum of patients with papillary thyroid carcinoma（ PTC） and its significance. Methods Reverse transcription polymerase chain reaction（ RT-PCR） was used to detect the expression levels of miR-21 in carcinoma tissues and serum of 43 cases of 46 patients with PTC,and also compared with 35 cases of nodular goiter and 18 healthy people. The correlations of miR-21 expression to the clinicopathological features and prognosis were analyzed. Results The relative content of miR-21 in carcinoma tissues and serum of 46 patients with PTC was 2. 74±1. 53 and 0. 21 ±0. 08,and it was 0. 92 ±0. 81 and 0. 08 ±0. 05 in carcinoma tissues and serum of 35 patients with nodular,0.86±0. 39 and 0. 07±0. 03 in normal tissues and serum of 18 healthy people. The expression of miR-21 in carcinoma tissues was consistent with that in serum. The expression of miR-21 in carcinoma tissues and serum of patients with PTC was higher than that of patients with nodular goiter and healthy people（ P〈0. 05）. The expression level of miR-21 had a correlation with extraglandular invasion,distant metastasis,lymphatic metastasis and TNM classification（ P〈0. 05）. Conclusion The expression of miR-21 in carcinoma tissues and serum of patients with PTC was higher than that of patients with nodular goiter and healthy people,and the up-regulated expression of serum miR-21 is correlated with the clinicopathologic features of PTC,indicating that miR-21 could be used as a potential marker for early detection of PTC.