苗药矮地茶的HPLC指纹图谱研究

Study on HPLC Fingerprint of Miao Medicine Ardisia japonica

目的:建立苗药矮地茶的HPLC指纹图谱分析方法。方法:采用HPLC法,Diamonsil C18柱(250 mm×4.6 mm,5μm),甲醇-0.1%磷酸溶液梯度洗脱,流速为1.0 m L/min,柱温35℃,检测波长为250 nm。结果:对10批贵州不同产地样品进行了分析,确定了32个共有峰,构成了矮地茶的HPLC指纹图谱,其中4个共有峰得到指认,分别为没食子酸、岩白菜素、绿原酸、槲皮苷。结论:此方法简便,准确,重复性好,可用于矮地茶的HPLC指纹图谱测定,为今后规范其药材资源及质量评价提供科学依据。

Objective： To establish the HPLC fingerprint of Ardisia japonica. Methods： HPLC analysis was performed on a Diamonsil C18column（ 250 mm × 4. 6 mm,5 μm） with the eluting system of gradient consisted of methanol-0. 1% H3PO4. The flow rate was 1. 0m L / min. The column temperature was maintained at 35 ℃ and the detection wavelength was 250 nm. Results： The HPLC fingerprint was established and 10 batches of samples with 32 common peaks were compared. Four peaks were identified as gallic acid,bergenin,chlorogenic acid and quercitrin. Conclusion： The method with good reproducibility is simple and accurate,which can be used for determination of HPLC fingerprint and quality control of Ardisia japonica. It provides scientific basis of future study of Ardisia japonica.