蜂胶总黄酮对STZ诱导糖尿病大鼠降血糖机制研究

Study on Hypoglycemic Effect and Mechanism of Total Flavonoids of Propolis in STZ Diabetic Rats

目的：研究蜂胶总黄酮（TFP）对链脲佐菌素（STZ）致糖尿病大鼠的降血糖作用及其机制。方法：采用喂养高脂饲料4 w后再腹腔注射STZ的方法造模,根据血糖值随机分为模型组及TFP高（240 mg/kg）、中（120 mg/kg）、低（60 mg/kg）剂量组,另设正常对照组,每组10只。期间TFP高、中、低剂量组灌胃给药1次/d,正常对照组与模型组灌胃给予等体积0.5%羧甲基纤维素钠（CMC-Na）,连续4 w。给药期间,糖尿病大鼠均继续喂以高脂饲料。每周测血糖1次,末次给药结束后,禁食12 h,乙醚麻醉,取肝组织测肝糖原,腹主动脉取血,检测各组大鼠血清中血糖（GLU）、胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL-C）、低密度脂蛋白（LDL-C）、糖化血红蛋白（GHb）、胰岛素（INS）、C肽（C-P）、超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽（GSH）、肿瘤坏死因子（TNF-α）、游离脂肪酸（FFA）及一氧化氮（NO）水平。结果：与正常对照组大鼠比较,模型组大鼠血清GLU、TG、TC、LDLC、MDA、FFA、TNF-α、GHb及NO水平显著升高（P〈0.05-P〈0.001）,血清HDL-C、SOD、GSH、INS、C-P及肝糖原含量显著降低（P〈0.01或P〈0.001）。与模型组比较,TFP高、中剂量对以上指标均有显著的改善作用（P〈0.05-P〈0.01）。结论：TFP对糖尿病大鼠具有显著的降血糖作用,可显著改善糖尿病大鼠糖脂代谢并抑制胰岛素抵抗。

Objective： To study hypoglycemic effect and mechanism of the total flavonoids of Propolis（ TFP） in the STZ diabetic rats. Methods： The model of type 2 diabetic rats was induced by high-fat diet feeding for 4 weeks combined with intraperitoneal injection of streptozotocin（ STZ）. Then the rats without above-mentioned treatment were selected as the normal control group,the diabetic rats were randomly divided into four groups by blood glucose,the model control group,high doses（ 240 mg / kg）,medium doses（ 120 mg / kg）and low doses（ 60 mg / kg） TFP groups. The TFP preparation was intragastric administration given to rats in TFP groups. The model control rats were treated intragastric administration with 0. 5% sodium carboxymethyl cellulose（ CMC-Na） for 4 weeks. The rats were fed with high fat diet during treatment. 12 hours after the last administration,glucose（ GLU）,total cholesterol（ TC）,triglyceride（ TG）,high density lipoprotein（ HDL-C）,low density lipoprotein（ LDL-C）,glycated hemoglobin（ GHb）,serum insulin（ INS）,C-peptide（ C-P）,superoxide dismutase（ SOD）,malondialdehyde（ MDA）,glutathione（ GSH）,tumor necrosis factor-α（ TNF-α）,free fatty acid（ FFA）,nitric oxide（ NO）,and hepatic glycogen were determined. Results： Compared with the normal group,the levels of GLU,TC,TG,HDL-C,FFA,TNF-α,NO,MDA and GHb in serum were increased significantly in the model control group（ P〈0. 05 - P〈0. 001）,the levels of HDL-C,INS,C-P,SOD and GSH in serum and hepatic glycogen in hepatic tissue were decreased significantly（ P〈0. 01 or P〈0. 001）. The levels of all above indexes of high and medium doses groups were improved significantly compared with the model control group（ P〈0. 05 - P〈0. 01）. Conclusion： TFP can significantly decrease the level of blood glucose,improve the glucose and lipid metabolism and inhibit insulin resistance in STZ diabetic rats.