NLRP3基因单核苷酸多态性与上海地区寻常痤疮患病率的关系分析

Analysis of the relationship between NLRP3 gene single nucleotide polymorphisms and the prevalence of acne vulgaris in Shanghai area

目的：探讨NLRP3基因单核苷酸多态性（SNP）与上海地区寻常痤疮患病率的相关性。方法：收集145例中、重度痤疮患者及158例健康人群的临床资料和血液标本，TaqMan探针法对rs10754558和rs4612666位点进行分型．ABI7900扫描分型结果，荧光定量PCR检测NLRP3基因表达水平。结果：中、重度痤疮患者rs10754558位点CC、CG、GG基因型频率分别为0．168、0．476和0．338，对照组为0．285、0．487和0．228，G等位基因的分布频率差异存在统计学意义（P=0．010．OR=1．522．95％CI=1．075-2．098）。痤疮患者与健康人群rs4612666位点基因型差异无统计学意义（P=0．708，OR=1．065，95％CI=0．767-1．479）。结论：NLRP3基因rs10754558位点与上海地区痤疮患病率存在相关性，而rs4612666位点与其无相关性。

Objective： To analyze the association of NLRP3 geue single nucleotide polymorphisms （SNP） with the risk of prevalence of acne vulgaris in Shanghai area. Methods： Clinical data and blood samples were collected from 145 patients with moderate to severe ache and 158 healthy controls. TaqMan assay was performed to determine the genotypes of two SNPs of rs10754558 and rs4612666 sites by ABI7900 scanning. Genomic mRNA of NLRP3 was detected by fluorescence quantitative polymerase chain reaction （PCR）. Results： The frequencies of CC, CG and GG genotypes of the SNP rs10754558 were 0.168, 0.476 and 0.338 in patients with aene, while they were 0.285, 0.487 and 0.228, respectively, in healthy controls. The frequency of G allele of the SNP rs10754558 between two groups was significantly different （P=0.010, OR=1.522, 95%CI= 1.075-2.098）. No significant differences were observed in the genotype distribution or allele frequency of the SNP rs4612666 between two groups （P=0.708, OR=1.065, 95%CI=0.767-1.479）. Conclusions： The prevalence of acne vulgaris in Shanghai area is likely associated with the SNP rs10754558 in the NLRP3 gene, but not the SNP rs4612666.