变性梯度凝胶电泳(DGGE)技术在畜禽粪便厌氧发酵液中的研究进展

Advances in anaerobic fermentation broth denaturing gradient gel electrophoresis(DGGE) technique in animal feces

变性梯度凝胶电泳技术的原理为DGGE原理。优点是:操作的简便性、快速同时对比分析大量样品、能跟其他分子生物学技术相结合。对变性梯度凝胶电泳技术在畜禽粪便厌氧发酵液中,微生物菌落的多样性和动态性研究,得出厌氧发酵系统是处理畜禽粪便的有效途径之一,而发酵液中各种微生物是维持系统稳定运行的关键因子,将DGGE技术应用到畜禽粪便厌氧发酵液中,可以用来分析不同时期发酵液中不同微生物优势菌群的种类及数量变化,指导厌氧系统的稳定运行。

Gradient gel electrophoresis DGGE principle principle degeneration. The advantages： ease of operation, quick comparative analysis of large numbers of samples at the same time, talk to other molecular biology techniques combined. Denaturing gradient gel electrophoresis in manure anaerobic fermentation broth, diversity and dynamics of microbial colonies, obtained anaerobic fermentation system is one of the effective ways to deal with animal manure, and a variety of fermentation broth microorganisms is a key factor to maintain the stability of the system, the DGGE technique will be applied to manure anaerobic fermentation broth can be used to analyze changes in the types and number of different periods in different fermentation microbial flora advantages, guiding stable anaerobic system run.