曲古抑菌素A对人Tenon囊成纤维细胞作用的研究

Effects of trichostatin A on human Tenon capsule fibroblast

目的：研究曲古抑菌素A（ trichostatin A,TSA）对体外培养的人Tenon囊成纤维细胞（ human Tenon capsule fibroblast, HTF）增殖能力以及组蛋白去乙酰化酶1（ histone deacetylase 1,HDAC1）和HDAC2蛋白表达的影响。方法：取青光眼滤过术中Tenon囊组织进行HTF体外培养。选取第3～6代细胞进行实验。设置空白对照组及TSA组（600nmoL／LTSA加入培养液中），分别于培养后1，2，3d应用MTF法检测细胞活力变化；以600nmol／LTSA处理HTF2d后，Westernblot法检测细胞中HDAC1和HDAC2蛋白的表达。结果：与对照组比较，TSA作用1d后HTF活力下降，呈时间依赖性，具有统计学差异（P〈0．05）。TSA处理HTF后2d，Westernblot法检测HDAC1和HDAC2蛋白表达受到明显抑制。结论：TSA可以通过抑制HDAC1和HDAC2的表达量，抑制人Tenon囊成纤维细胞增殖，从而减少术后结膜瘢痕形成可能。

AIM：To investigate the effect of trichostatin A （ TSA） on cell proliferation and the expressions of histone deacetylase 1 （ HDAC1 ） and HDAC2 in cultured human Tenon capsule fibroblast （ HTF） . METHODS： Human Tenon capsule fibroblasts were cultured in vitro after glaucoma filtration surgery, The third to sixth passage of cell were treated by 600nmol/L TSA or none. Cell viability measured by Ml-r assay after 1, 2 and 3d respectively. The expressions of HDAC1 and HDAC2 were analyzed by Western blot 2d after TSA treatment. RESULTS： Compared to the control, cell viability decreased significantly after treatment with TSA at ld （ P〈 0.05）, presented time-dependent manner. The expression of HDAC1 and HDAC2 significantly reduced in TSA-treated HTF compared with control cells at 2d after TSA treatment. CONCLUSION： TSA inhibits the proliferation of Tenon capsule fibroblast by inhibiting the expression of HDAC1 and HDAC2, and reduces subconjunctival scar formation.