小鼠糖皮质激素诱导的肿瘤坏死因子受体相关蛋白生物信息学分析

Bioinformatic analysis of mouse glucocorticoid-induced tumor necrosis factor receptor-related protein

目的获取小鼠糖皮质激素诱导的肿瘤坏死因子受体相关蛋白（GITR）氨基酸序列,并预测其结构和功能。方法利用网络平台及相关生物学软件对小鼠GITR氨基酸序列进行生物信息学分析。结果小鼠GITR氨基酸序列与人等其他物种具有56%的同源性,具有信号肽、胞外区、跨膜区及胞内区等结构;小鼠GITR蛋白胞外区位于第22-153号氨基酸序列区间;可能含有4个N-糖基化位点、4个丝氨酸磷酸化位点、1个苏氨酸磷酸化位点以及1个酪氨酸磷酸化位点。结论小鼠GITR氨基酸序列的生物信息学分析为进一步表达该蛋白及其相关功能研究奠定了基础。

Objective To obtain the amino acid sequence of glucocorticoid-induced tumor necrosis factor receptor-related protein（ GITR） of mouse with an attempt to predict the structure and function of GITR. Methods The amino acid sequence of GITR was analyzed by bioinformatic methods using the network platforms and some bioinformatic softwares. Results The amino acid sequence of mouse GITR showed a homology of 56% with that of other species,including human. GITR protein was composed of signal peptide,extracellular region,transmembrane domain and intracellular region. The extracellular region of mouse GITR is located at the position of 22-153 amino acid residues. The sequence of mGITR might contain four N-glycosylation sites,four serine phosphorylation sites,one threonine phosphorylation site and one tyrosine phosphorylation site. Conclusion The bioinformatic analysis of mouse GITR could provide a basis for the further expression and functional study of mouse GITR protein.