阿维A对P物质诱导的HaCaT细胞NF-κB表达的影响

Effects of acitretin on expression of NF-κB in HaCaT cells inducted by substance P

目的:明确阿维A对HaCaT细胞NF-κB表达的影响。方法:细胞共分5组:对照组、单纯P物质刺激组、P物质+小剂量阿维A组、P物质+中剂量阿维A组、P物质+大剂量阿维A组。应用免疫细胞化学法、Western-blot和RT-PCR法检测各组HaCaT细胞中NF-κB阳性细胞表达率、细胞蛋白和基因表达的变化。结果:单纯P物质刺激组较对照组阳性细胞表达率、细胞蛋白、mRNA表达均增强。各阿维A剂量组较P物质组阳性细胞表达率、蛋白和mRNA表达均降低(P<0.05或P<0.01)。结论:阿维A对人角质形成细胞NF-κB的调控是其治疗皮肤病的作用途径之一。

Objective：To determine the effects of acitretin on the expression in NF-κB of HaCaT cells.Methods：The cells were classified into control group,SP group,group of low dose acitretin,group of moderate dose acitretin and group of larger dose acitretin.The expressions of NF-κB in positive cells,cell protein and mRNA were detected by immunocytochemical technique,Western-blot method and RT-PCR method,respectively.Results：Compared with the control group,the expression rate of positive cells,the relative expression value of NF-κB mRNA and cell protein were significantly increased in SP group （P＜0.05）and was significantly decreased in acitretin groups （P＜0.05 or P＜0.01）.Conclusion：Acitretin is effective in the treatment of some skin diseases by regulating the expression of NF-κB in keratocytes.