NDRG2基因亚型对卵巢癌HO-8910细胞增殖的影响

The proliferation effect of N-myc downstream regulated gene 2 subtypes mediated by adenovirus on the ovarian cancer HO-8910 cells

目的:构建表达N-myc下游调节基因2(NDRG2)两种亚型(长短2种亚型分别命名为NDRG2L、NDRG2S)的重组逆转录病毒载体,观察逆转录病毒介导的NDRG2基因对卵巢癌HO-8910细胞增殖抑制的影响。方法:以携带人NDRG2基因的两种亚型逆转录病毒载体转染体外培养的卵巢癌细胞株HO-8910。采用Western blot检测目的基因的表达,MTT、平板克隆实验检测对细胞增殖能力的影响。结果:细胞经逆转录病毒转染后,Western blot检测NDRG2两亚型蛋白特异表达。MTT、平板克隆实验结果显示NDRG2基因两种亚型对HO-8910细胞生长均有明显促进作用(P<0.05),而二者之间无明显差别。结论:逆转录病毒载体使卵巢癌细胞表达NDRG2基因,可以明显促进HO-8910细胞的生长和增殖。

Objective： To construct p LNCX2- NDRG2,p LNCX2- NDRG2 S recombinant retroviral vector and to observe its effect on the proliferation of ovarian cancer HO- 8910 cells. Methods： The p LNCX2- NDRG2 L,p LNCX2- NDRG2 S were transfected into ovarian cancer HO- 8910 cells,and the protein expression of NDRG2 was detected by Western blot. The effect on the proliferation of cells were detected by MTT and plate cloning test. Results： The NDRG2 L,NDRG2S protein was efficiently expressed in ovarian cancer HO- 8910 cells. MTT and plate clone experimental results showed that the 2 isoforms of NDRG2 gene significantly promote the growth of the HO- 8910 cells（ P 0. 05）,but no significant difference between the two isoforms. Conclusion： The growth and proliferation of ovarian cancer HO- 8910 cells were significantly promoted by NDRG2 L and NDRG2S.