白骨壤果实中抗氧化活性成分研究

Antioxidative Chemical Constituents from the Fruits of Avicennia marina

【目的】为获得高效的抗氧化先导化合物,对红树白骨壤（犃狏犻犮犲狀狀犻犪犿犪狉犻狀犪）果实中化学成分进行研究.【方法】采用柱色谱和高效液相色谱及细胞定量分析方法,对白骨壤果实中抗氧化活性成分进行研究,并运用色谱分析和文献对照方法,鉴定其化学结构.【结果】从白骨壤果实中分离鉴定了4个具有抗氧化活性的单体化合物,分别为tamarixetin-3-O-β-D-glucopyranoside（1）、3,4-dihydroxyphenethylalcohol-6-O-caffeoyl-β-D-glucoside（2）、类叶升麻苷（3）、焦地黄苯乙醇甙C（4）.化合物1～4的EC50值分别为（16.0±0.98）μM,（18.5±1.47）μM,（9.5±1.09）μM 和（12.0±0.89）μM.【结论】红树白骨壤果实中存在着具有强抗氧化活性成分（1～4）,且上述活性成份均为首次从该物种中分离获得.

Objective]In order to obtain strong antioxidant compounds,the fruits of mangrove Avicennia marina were investigated.[Methods]The antioxidative constituents were isolated and analyzed by column chromatograph,HPLC and cell quantiative analysis.The structure of the constituents was identified by chromatograph analysis results and previous paper.[Re-sults]The fruits of mangrove Avicennia marina contains one flavonoid glycoside,tamarixetin-3-β-D-glucopyranoside (1),and three known phenylethyl glycosides,3,4-dihydroxyphen-ethylalcohol-6-O-caffeoyl-β-D-glucoside (2),acteoside (3)and jionoside C (4).The EC50 values of compounds 1~4 were (16.0±0.98)μM,(18.5±1.47)μM,(9.5±1.09)μM,and (12.0±0.89)μM,respectively.[Conclusion]The strong antioxidant compounds were obtained from the fruits of Avicennia marina,and these compounds were obtained from the fruits of this species for the first time.