拟南芥ACOS5基因启动子的克隆和表达载体构建

Cloning ACOS5 Gene Promoter of Arabidopsis and Construction of Expression Vector

花药组织特异性启动子在花药发育的分子遗传学中有重要的研究价值.采用PCR方法克隆了1kb长度的ACOS5基因启动子,并将其连入含有GFP基因的拟南芥表达载体p1300中.电激转化农杆菌GV3101后,通过农杆菌介导转化拟南芥,获得转基因植株.显微观察显示,转基因植物花药只在绒毡层中有荧光表达.这说明ACOS5基因启动子可以在拟南芥花药中驱动外源GFP基因的特异性表达.

Anther-specific promoters play a significant role in molecular and genetic studies of the anthers development.In this study,the 1kb ACOS5 promoter was cloned by PCR,and fused into the Arabidopsis expression vector-p1300 containing GFPgene.Then it was transferred into Agrobacterium-tumefaciens GV3101 by electroporation.The transgenic Arabidopsis plants were obtained through Agrobacterium mediated transformation.The microscopic observation showed that green fluorescence only occurred in tapetum of transgenic plant anthers.It suggested that ACOS5 gene promoter could drive specific expression of exogenous GFPgene in the Arabidopsis anthers.