CXCL12和其受体CXCR4在儿童造釉细胞型颅咽管瘤的表达及意义

The expression and significance of CXCL12 and its receptor CXCR4 in children with adamantinomatous craniopharyngioma

目的探讨CXCL12、CXCR4与Ki-67在儿童造釉细胞型颅咽管瘤的表达与肿瘤复发及预后的相关性。方法收集45例儿童造釉细胞型颅咽管瘤标本,其中复发27例,非复发18例。应用高通量基因芯片、荧光实时定量PCR和免疫组织化学法对标本的CXCL12、CXCR4和Ki-67表达进行检测及验证。结果基因芯片结果:复发样本中CXCL12和CXCR4基因显著上调。CXCL12和CXCR4蛋白的高表达与复发明显相关(P<0.05),而与肿瘤大小和性别无关(P>0.05)。CXCR4与CXCL12蛋白表达呈正相关(r=0.74,P<0.01),CXCR4与Ki-67表达呈弱相关(r=0.28,P=0.06),而CXCL12和Ki-67的表达无相关性(r=0.15,P=0.32)。结论 CXCL12和CXCR4高表达可能是评估儿童造釉细胞型颅咽管瘤复发的肿瘤标志物。

Objective To explore the expression of CXCL12, CXCR4 and Ki-67 in children with adamantinomatous craniopharyngioma and their correlation with the recurrence and prognosis of tumor. Methods Samples of 45 child patients with adamantinomatous craniopharyngiomas were collected, including recurrent tumors in 27 and nonrecurrent tumors in 18. The expression of CXCL12, CXCR4 and Ki-67 mRNA and protein were detected by high-throughput microarray analysis, quantitative real-time PCR （qRT-PCR） and immunohistochemistry. Results The microarray results showed that the expressions of CXCL12 and CXCR4 genes were significantly increased in recurrent samples. The high level of CXCL12 and CXCR4 were significantly associated with recurrence （P 〈 0.05） and not associated with tumor size and gender （P 〉 0.05）. There was positive correlation between CXCR4 and CXCL12 proteins （r = 0.74, P 〈0.01）. A weak association was found between CXCR4 and Ki-67 proteins （r = 0.28, P = 0.06）, while the expression of CXCL12 and Ki-67 proteins were not significantly correlated （r = 0.15, P = 0.32）. Conclusion The high levels of CXCL12 and CXCR4 may be the tumor marker for evaluating the recurrence in children with adamantinomatous craniopharyngiomas.