肌肉注射人脐带间充质干细胞健康大鼠心肌组织ki-67、phh3及cTnT的表达

Effects of human umbilical cord mesenchymal stem cells via intramuscular injection on the myocardial ki-67, phh3 and cTnT expression in normal rats

背景:肌肉注射人脐带间充质干细胞是否会增加正常心肌细胞的再生能力,对正常心肌是否会产生不良影响,尚不明确。目的:观察肌肉注射人脐带间充质干细胞对健康Wistar大鼠心肌组织中ki-67、phh-3及cTnT表达的影响。方法:将60只健康雄性Wistar大鼠随机分为正常组,培养液组,上清液组,脐带间充质干细胞低、中、高剂量组,每组分别给予大鼠四肢肌肉注射不同液体,分别为:PBS、DMEM培养液、培养人脐带间充质干细胞的上清液、0.25×105人脐带间充质干细胞、1.0×105人脐带间充质干细胞、4.0×105人脐带间充质干细胞,间隔4周共注射2次,第8周处死大鼠,取心肌组织固定、包埋及切片,应用免疫组织化学方法检测大鼠心肌组织中ki-67、phh-3及cTnT的表达。结果与结论:正常组中ki-67在心肌组织细胞胞核呈阴性表达,phh3在心肌组织细胞胞核呈弱阳性表达,cTnT在心肌细胞胞浆中呈强阳性表达;与正常组相比,培养液组、上清液组及脐带间充质干细胞低、中、高剂量组间ki-67、phh-3及cTnT的表达差异无显著性意义(F=1.076、0.167及0.300;P>0.05)。提示经肌肉注射人脐带间充质干细胞或其培养上清液对健康Wistar大鼠心肌组织ki-67、phh-3及cTnT的表达无影响。

BACKGROUND：It is unclear whether intramuscular injection of human umbilical cord mesenchymal stem cells wil increase regeneration of normal myocardial cells and play adverse effects on normal myocardium. OBJECTIVE：To observe the effects of intramuscular injection of human umbilical cord mesenchymal stem cells on expression of the myocardial ki-67, phh3 and cTnT in normal Wistar rats. METHODS：A total of 60 male Wistar rats were divided into six groups randomly：normal group, solution group, supernatant group, low concentration group, moderate concentration group, and high concentration group. These groups were intramuscularly injected different liquid, respectively, as fol ows：PBS, DMEM, the supernatant of human umbilical cord mesenchymal stem cells, human umbilical cord mesenchymal stem cells with amount of 0.25×10^5, human umbilical cord mesenchymal stem cells with amount of 1.0×10^5, human umbilical cord mesenchymal stem cells with amount of 4.0×10^5 . After 4 weeks, each rats received the second same intramuscular injections of human umbilical cord mesenchymal stem cells. Four weeks after the second injection, al rates were kil ed to obtain myocardial tissues which were fixed, embedded and sectioned. Final y, the ki-67, phh3 and cTnT expressions of the myocardium were detected by immunohistochemical technique. RESULTS AND CONCLUSION：In the normal group, negative expression of the ki-67 was observed in the caryon of myocardial cells, weak positive expression of the phh3 was observed in the caryon of myocardial cells, and positive expression of the cTnT was observed in the caryon of myocardial cells. Compared with the normal group, the expression of the ki-67, phh3 and cTnT in the myocardium had no significant difference among the other groups （F=1.076, 0.167, 0.300;P〉0.05）. Human umbilical cord mesenchymal stem cells or the supernatant of human umbilical cord mesenchymal stem cells via intramuscular injection have no effects on the expression of the ki-67, phh3 and cTnT in normal Wistar rats.