纳米银-表皮生长因子促进大鼠糖尿病创面愈合

The sustained-release carrier of Nanosilver-epidermal growth factor in promoting wound healing of diabetes

目的 探讨纳米银-表皮生长因子缓释复合体的抗菌效果和治疗大鼠糖尿病难愈性创面的疗效.方法 依照前期方法制备出纳米银-表皮生长因子缓释复合体,设计纳米银-表皮生长因子缓释复合体组、单纯纳米银组、单纯生长因子组、单纯生理盐水对照组、青霉素钠阳性对照组,对比检测各组对5种常见微生物（金黄色葡萄球、大肠埃希菌、铜绿假单胞菌、白色念珠菌、肺炎克雷伯菌）的抗菌活性;然后将糖尿病大鼠创面模型随机分为五组,分别以纳米银-生长因子缓释复合体、纳米银及表皮生长因子联合应用、单纯表皮生长因子、单纯纳米银、生理盐水治疗创面,不同时相观察各组创面愈合情况.结果 缓释复合体及纳米银抗菌性能明显高于其它三组,同时缓释复合体能显著缩短糖尿病大鼠创面愈合时间（P＜0.05）;第3天各组愈合率差异无统计学意义（P＞0.05）,第7天和13天缓释复合体的愈合率[（61.71±8.78）％、（100±2.60）％]明显高于其他四组（P＜0.05）.结论 纳米银-表皮生长因子缓释复合体不仅具有良好的抗菌性,且能快速促进糖尿病创面的愈合.

Objective To investigate the antibacterial properties and curative effects of a nanosilver-epidermal growth factor （NanoAg-EGF） sustained-release carrier.Methods First,in accordance with former method,sustained-release carrier of NanoAgEGF was prepared,with the design of the NanoAg-EGF group,NanoAg-alone group,EGF-alone group,control groups of normal saline-alone,and benzylpenicillin sodium.Contrasting detection on antibacterial properties of five kinds of common microbes （staphylococcus aureus,escherichia coli,pseudomonas aeruginosa,candida albicans,and streptococcus pneumonia） in each group was used to confirm their antibacterial properties.Then,wound models of diabetic rats were randomly divided into five groups,with the wound treatments by sustained-release cartier of NanoAg-EGF,combined application of NanoAg ＋ EGF,EGF-alone,NanoAg-alone,and normal saline-alone control group,respectively.The observation on the healing situation of each group in different times was used to ensure the curative effect.Results In the antibacterial experiments,it was found that the NanoAg-EGF and NanoAg had strong antibacterial properties.Their antibacterial properties had no significant difference （P 〉 0.05）.The control group,penicillin sodium only had relatively weak antibacterial properties on staphylococcus aureus and streptococcus pneumoniae,with obviously weaker function compared to the NanoAg-EGF and NanoAg groups （P 〈 0.05）.The control groups,EGF-alone and normal saline-alone had no inhibitor function on each kind of pathogenic microorganisms.In the wound healing experiments on diabetic rats,it was found that NanoAgEGF shortened significantly the wound healing time of diabetic rats （P 〈 0.05）.On the 3rd day,the healing rate of each group had no difference （P 〉 0.05）.On the 7th day and 13th day,the healing rates of NanoAg-EGF [（61.71 ± 8.78）% and （100 ± 2.60） %] were significantly higher than the other four groups （P 〈 0.05）.Conclusions NanoAg-EGF not only has good antibacterial properties but also can promote rapidly the wound healing of diabetes.