IgA肾病患者血浆褪黑素水平与氧化应激的关系

Relationship of Blood Plasma Melatonin Levels and Oxidative Stress in Patients with IgA Nephropathy

目的:探讨IgA肾病肾功能正常患者血浆褪黑素水平与氧化应激的关系,为褪黑素用于治疗IgA肾病提供科学依据。方法:分为健康对照组(n=22)及IgA肾病肾功能正常组(n=48)。采用酶联免疫吸附法(ELISA)测定各组血浆褪黑素水平及分光光度法测定各组血浆丙二醛(MDA)、超氧化物歧化酶(SOD)水平。结果:与正常对照组相比,IgA肾病肾功能正常组患者血浆褪黑素降低[(64.05±29.23)ng/L vs.(80.54±35.12)ng/L,P<0.05],而血浆MDA水平升高[(45.17±26.47)U/ml vs.(31.14±26.00)U/ml,P<0.05],血浆SOD水平下降[(76.25±19.34)U/ml vs.(86.71±18.55)U/ml,P<0.05)。进一步分析结果显示,IgA肾病患者血浆褪黑素水平与MDA水平呈负相关(r=-0.308,P<0.05),与SOD水平呈正相关(r=0.321,P<0.05)。结论:IgA肾病肾功能正常患者血浆褪黑素水平较健康人降低,血浆MDA水平随血浆褪黑素水平降低而升高,血浆SOD水平随血浆褪黑素水平降低而降低。提示血浆褪黑素水平的降低引起机体抗氧化能力的下降,进而参与了IgA肾病的进展。补充外源性褪黑素可能为治疗IgA肾病具有潜力的方法。

Objective：To study the relationship of blood plasma melatonin levels and oxidative stress in patients with IgA nephropathy,and provide a basis for the treatment of IgA nephropathy with melatonin. Methods：Blood plasma melatonin levels and MDA levels,SOD levels were measured by using ELISA and spectrophotography in 48 no renal failure IgA nephropathy patients,and in 22 normal people（control group）. Results：Compared with control group,blood plasma melatonin levels in no renal failure IgA ne-phropathy patients significantly decreased[（64. 05 ± 29. 23）ng/ L vs. （80. 54 ± 35. 12）ng/ L,P 〈 0. 05],MDA levels increased [（45. 17 ± 26. 47）U/ ml vs.（31. 14 ± 26. 00）U/ ml,P 〈 0. 05],and SOD levels decreased[（76. 25 ± 19. 34）U/ ml vs.（86. 71 ± 18. 55）U/ ml,P 〈 0. 05]. Blood plasma melatonin levels in no renal failure IgA nephropathy patients is in negative correlation to plasma MDA levels（r = - 0. 308,P 〈 0. 05）,and is in positive correlation to plasma SOD levels（r = 0. 321,P 〈 0. 05）. Conclu-sion：Blood plasma melatonin levels decreased in no renal failure IgA nephropathy patients. Reduction of blood plasma melatonin lev-els in no renal failure IgA nephropathy patients might lead to elevation of plasma MDA and reduction of plasma SOD. Supplement of exogenous melatonin might be a promising treatment for IgA nephropathy in patients.