摘要
目的:研究不同基因型沙眼衣原体(CT)女性生殖道感染与临床表现的相关性;比较不同基因型CT感染宫颈分泌物中衣原体热休克蛋白60(c HSP60)表达的差异。方法:收集女性CT感染标本60例,巢式PCR扩增外膜蛋白Omp1基因的VS1-VS2序列,扩增产物通过基因测序进行分型。ELISA检测不同基因型沙眼衣原体感染宫颈分泌物中c HSP60表达,分析不同基因型沙眼衣原体感染与临床表现的相关性。结果:60例女性生殖道CT感染阳性标本中E型14例(23.33%),J型14例(23.33%),F型13例(21.67%),G型9例(15%),D型5例(8.33%),不确定型5例(8.33%)。女性生殖道J型CT感染患者宫颈分泌物中c HSP60表达均明显高于E型、F型、G型及D型(P值均<0.05);E型c HSP60水平高于G型,差异有统计学意义(P<0.05)。女性泌尿生殖道不同基因型CT感染者间的临床表现差异无统计学意义(2=2.42,P>0.05)。结论:女性生殖道CT感染患者中不同基因型CT感染与临床表现之间缺乏关联性,结果尚需扩大样本量进一步确定。
Objective：To analyze the correlation between CT infection with different genotypes and clinical manifestations , and observe cHSP60 expression of cervical secretion in women with CT infection.Methods：VS1-VS2 sequence of major outer membrane protein was amplified by nes-ted PCR in the clinical specimens from 60 women with CT infection , followed by gene sequencing . Expression of cHSP 60 in cervical secretion was detected by ELISA .The correlation between infec-tion of different CT genotypes and clinical manifestations was analyzed .Results：Of 60 CT positive samples, serovars E, J, F, G, D and unidentified were present in 14 （ 23.33%）, 14 （23.33%）, 13 （21.67%）, 9 （15%）, 5 （8.33%）, and 5 （8.33%） cases respectively.Ex-pression of cHSP60 increased significantly in J serovar compared with other serovars in cervical se-cretion （P〈0.05）.Expression of cHSP60 was higher in E genotype than that in G genotpye （P〈0.05 ） .There was no correlation between different CT genotypes and clinical manifestations in women with genital CT infection （ P〉0.05） .Conclusion：There could be no correlation between different CT genotypes and clinical manifestations in women with genital CT infection , but it de-serves to study on large sample size .
出处
《皮肤性病诊疗学杂志》
2014年第5期375-379,共5页
Journal of Diagnosis and Therapy on Dermato-venereology
