下调miR-214对鼻咽癌CNE2细胞凋亡的影响

Effects of Down-Regulation of miR-214 on the Apoptosis of CNE2 Cell in Nasopharyngeal Carcinoma

目的:研究miR-214对鼻咽癌细胞CNE2凋亡的影响及机制。方法:RT-PCR检测鼻咽癌细胞CNE2和正常鼻咽上皮细胞NP69细胞中miR-214的表达;通过miR-214inhibitor转染鼻咽癌细胞CNE2,流式细胞仪(FCM)检测鼻咽癌细胞凋亡变化;并用Western印迹法检测细胞中Bcl-2和Bax蛋白的表达变化。结果:正常鼻咽上皮细胞明低表达miR-214,而鼻咽癌CNE2细胞中明显高表达miR-214(P<0.05),miR-214inhibitor可浓度依赖性的抑制miR-214的表达(P<0.05)。40μmol/L和5μmol/L的miR-214inhibitor组细胞凋亡率分别为:(45.3±9.1)%和(12.3±3.8)%,而NC inhibitor组细胞的凋亡率为(5.1±0.4)%。miR-214inhibitor可下调Bcl-2的表达(P<0.05),对Bax的表达无明显影响。结论:miR-214可能通过下调Bcl-2的表达从而诱导CNE2细胞凋亡,这可能为鼻咽癌的治疗提供新靶点。

Objective：To investigate the effect of MicroRNA-214（miR-214）on apoptosis of nasopharyngeal carcinoma cell line CNE2.Methods：miR-214 in vitro was transiently transferred into nasopharyngeal carcinoma cell line CNE2.The expression level of miR-214 in CNE2cells and nasopharyngeal epithelial cell line NP69 was detected by real-time fluorescence quantitative PCR.Apoptosis was analyzed by flow cytometry.Expression of Bcl-2and Bax protein was detected by Western blot.Results：miR-214 in nasopharyngeal carcinoma cell line CNE2 was significantly increased compared with nasopharyngeal epithelial cell line NP69（P〈0.05）.The apoptosis rate of40μmol/L miR-214 inhibitor group,5μmol/L miR-214 inhibitor group and NC inhibitor group were（45.3±9.1）%,（12.32±3.8）%and（5.12±0.4）%,respectively.The difference was significant statistically（P〈0.05）.And the expression levels of Bcl-2were markedly down-regulated by miR-214inhibitor（P〈0.05）.Conclusion：The miR-214down-regulated Bcl-2induces apoptosis in nasopharyngeal carcinoma cells,which provids a new target for the treatment of nasopharyngeal carcinoma.