雌二醇经丝苏氨酸蛋白激酶激活核因子κB通路对子宫内膜癌Ishikawa细胞增殖迁移的影响

Estradiol enhances the proliferation and migration of Ishikawa cells by promotion of angiogenesis induced by activation of NF-κB via AKT pathway

目的 探讨雌二醇在子宫内膜癌Ishikawa细胞中是否通过丝苏氨酸蛋白激酶（AKT）介导核因子κB （NF-κB）通路产生细胞因子,及其对细胞增殖及迁移的影响.方法 Western blot法检测雌二醇作用Ishikawa细胞后AKT活化情况,以及AKT和ER抑制剂对雌二醇活化AKT的影响,TransAM NF-κB p65检测不同浓度雌二醇及1×10^-6 mol/L雌二醇作用不同时间后的NF-κB活性水平.经雌二醇（雌二醇组）、ER抑制剂（ER组）、AKT抑制剂（AKT组）和NF-κB抑制剂（NF-κB组）分别预处理Ishikawa细胞后,采用荧光定量PCR检测细胞内血管内皮生长因子（VEGF）、碱性成纤维细胞生长因子（bFGF） mRNA表达,Western blot法检测VEGF、bFGF蛋白的变化,流式细胞仪检测细胞周期的变化,羟基荧光素二醋酸盐琥珀酰亚胺脂（CFSE）法检测细胞增殖能力,Transwell检测细胞迁移能力.结果 Western blot检测结果显示,雌二醇、AKT和ER抑制剂作用Ishikawa细胞后AKT活性水平分别为0.090±0.075、0.013 ±0.036、0.042±0.008,对照组为0.053±0.036;雌二醇组的AKT活性值比对照组、ER组及AKT组升高,差异均有统计学意义（P＜0.05）.TransAM检测显示,雌二醇浓度为1×10^-6mol/L时,Ishikawa细胞NF-κB活性为0.77±0.20,较对照组（0.51±0.16）明显升高,差异有统计学意义（P＜0.05）;1×10^-6mol/L雌二醇作用30 min及1h时,Ishikawa细胞NF-κB活性水平最高（P＜0.05）.AKT组NF-κB活性为0.54±0.27,低于1×10^-6mol/L雌二醇组（P＜0.05）.雌二醇组VEGF、bFGF mRNA的表达分别为6.34±0.45和1.58 ±0.12,明显高于对照组的0.83±0.03和0.34±0.02（均P＜0.01）;雌二醇组VEGF、bFGF蛋白的表达均明显高于对照组、ER组、AKT组和NF-κB组（均P＜0.05）.雌二醇作用Ishikawa细胞后,细胞增殖数明显增多,G0/G1期比例明显低于对照组（均P＜0.01）.结论 雌二醇可能经AKT激活NF-κB通路产生VEGF和bFG因子,促进Ishikawa细胞的增殖与迁移能力。

Objective The aim of this study was to explore whether estradiol induces the expression of VEGF and bFGF in the endometrial cancer Ishikawa cells by activation of NF-κB via AKT pathway,and its effect on cell proliferation.Methods Western blot was used to detect the AKT protein expression in Ishikawa cells after stimulation with estradiol,and the effect of AKT inhibitor or ER inhibitor on the activation of AKT.TransAM kit was used to detect the NF-κB p65 activity.qPCR and Western blot were used to detect the expression of VEGF and bFGF mRNA and proteins in the Ishikawa cells after estradiol treatment （E2 group）,and pretreated with AKT inhibitor （AKT group） or ER inhibitor （ER group） or NFκB inhibitor （NF-κB group）,following the estradiol treatment.Flow cytometry and CFSE （carboxyfluorescein diacetate,succinimidyl ester） staining were used to examine the cell proliferation.Transwell was used to detect the migration ability of Ishikawa cells.Results Expression of p-AKT protein in the Ishikawa cells was markedly higher than that in the control group （P 〈 0.05）.Expressions of p-AKT protein in the AKT and ER groups were significantly decreased than that in the E2 group （P 〈 0.05）.The NF-κB activity was highest after stimulation with 1 × 10^-6 mol/L estradiol for 30 min to 1 h.AKT inhibitor significantly reduced the NF-κB activity （P 〈 0.05）.The expressions of VEGF and bFGF mRNA and proteins in the E2 group were significantly increased than that in the control group （P 〈 0.05）,and their expression in the AKT,ER and NF-κB groups were significantly decreased than that in the E2 group （P 〈 0.05）.The proliferation and migration abihties of the Ishikawa cells were significantly increased after estradiol stimulation.Conclusions Estradiol induces the production of VEGF and bFGF through activating NF-κB via AKT pathway,and enhances the proliferation and migration ability of cancer cells.