宫内生长受限大鼠胃黏膜nesfatin-1/NUCB2和ghrelin的表达及其意义

Expression of nesfatin-1/NUCB2 and ghrelin in gastric mucosa of rats with intrauterine growth retardation

目的探讨nesfantin-1、ghrelin在宫内生长受限(IUGR)大鼠胃黏膜的表达及其意义。方法采用母鼠妊娠期低蛋白饮食法,使其自然分娩建立IUGR新生大鼠模型。根据有无追赶生长分为追赶生长组(RC组)、无追赶生长组(RR组)、对照组(CC组)。应用实时荧光定量PCR技术及Western blot法测定不同日龄(12d、21d、28d)及追赶生长情况的SD大鼠胃黏膜的nesfantin-1/NUCB2、ghrelin mRNA及蛋白的表达。结果大鼠出生后胃黏膜即有nesfatin-1/NUCB2 mRNA及蛋白的表达。随日龄增长,3组仔鼠胃nesfatin-1/NUCB2 mRNA及蛋白的表达均明显升高,断奶前(12d、21d),RC组均高于CC组(均P<0.05),而断奶后(28d)RC组与CC组无差异;RR组始终低于RC组(均P<0.05)。生后12d开始RC组ghrelin mRNA及蛋白高于CC组,断奶后两组无差异;RR组于生后12d开始始终低于RC组及CC组。结论 Nesfatin-1与ghrelin共同表达于IUGR大鼠胃黏膜,参与长期的营养调控。

Objective To investigate the expression of nesfatin-1/NUCB2 and ghrelin in the gastric mucosa of rats with intrauterine growth retardation （IUGR） and its signiifcance. Methods The IUGR animal model was established by feeding rats low-protein diets during their pregnancy. Newborn rats were divided into catch-up growth, non-catch-up growth and control groups. Protein and mRNA levels of nesfatin-1/NUCB2 and ghrelin in the gastric mucosa of rats were determined by RT-PCR and Western blot, respectively. Results Nesfatin-1/NUCB2 mRNA and protein were expressed in the gastric mucosa of rats immediately after birth, and their expression increased in an age-dependent manner in all three groups. Furthermore, the level of nesfatin-1/NUCB2 in the catch-up growth group was higher than that in the control group before weaning, whereas there was no signiifcant difference in nesfatin-1/NUCB2 expression between the two groups after weaning. The level of nesfatin-1/NUCB2 in the non-catch-up growth group was lower than that in the catch-up growth group during the whole observation period. The level of ghrelin in the catch-up growth group was higher than that in the control group starting from day 12 after birth, whereas there was no signiifcant difference in ghrelin expression between the two groups after weaning. The level of ghrelin in the non-catch-up growth group was lower compared with those in the catch-up growth and control groups from days 12 to 28 after birth. Conclusions Nesfatin-1 and ghrelin are co-expressed in the gastric mucosa of rats with IUGR after birth and interact with each other to produce long-term nutritional regulation.