WT1在子宫内膜腺癌中的表达及其临床意义

The expression and clinical significance of WT1 in endometrial carcinoma

目的:探讨Wilms肿瘤基因(WT1)在子宫内膜腺癌组织、子宫内膜不典型增生组织以及正常内膜组织中的蛋白和基因表达水平及其临床意义。方法:采用实时荧光定量PCR及2-ΔΔCt法定量分析WT1 m RNA的相对表达量,应用蛋白印迹(Western blot)法检测子宫内膜组织中WT1蛋白的表达。结果:WT1在子宫内膜腺癌组织中的基因和蛋白表达均明显高于子宫内膜不典型组织和正常内膜组织(均P<0.01);WT1 m RNA与子宫内膜腺癌患者年龄、绝经情况、临床分期、淋巴结浸润情况、雌激素受体(ER)和体质量指数(BMI)无显著相关性,与病理分级、肌层浸润情况和孕激素受体(PR)相关(P<0.05);WT1蛋白的表达与患者年龄、绝经情况、临床分期、肌层浸润、ER、PR和BMI无显著相关性,与病理分级、淋巴结转移情况相关(P<0.05)。结论:子宫内膜腺癌中存在WT1 m RNA和蛋白表达水平上调,可能与子宫内膜腺癌的发生和进展有关。

Objective： To detect the protein and gene expression of WT1 in different types of tissues, in-cluding endometrial adenocarcinoma （EA） tissues, endometrial hyperplasia tissues and normal tissues and cor- related the expression with the clinicopathological features. Methods： The relative expression WT1 mRNA was detected by real-time quantitative PCR. The expression of WT1 protein was detected by Western blot in different types of endometrial tissues. Results： The expression of WT1 mRNA and protein in endometrial adenocarci- noma tissue was significantly higher than that in endometrial hyperplasia tissues and normal endometrial tissue respectively （P〈0.01）. There was no significant difference among age, menopause status, clinical stage, lymph node metastasis status, ER, BMI except histological stage, myometrial invasion and PR status concerned to WT1 gene expression （P〈0.05）. There were no significant correlations among the protein expressionof WT1 and age, menopause status, clinical stage, myometrial invasion ER, PR, BMI （P〉0.05）, but related to histological stage and lymph node status, significantly （P〈0.05）. Conclusion： Up-regulation of WT1 protein and gene expressionare correlated to the oncogenesis and progression of EA.