鄂西北地区茅苍术HPLC指纹图谱研究

Study on HPLC Fingerprints of Atractylodes Lancea in Northwest of Hubei Province

目的:建立鄂西北地区苍术药材HPLC指纹图谱,为苍术药材质量控制及品种鉴定提供依据。方法:采用HPLC法,以Diamonsil2#C18(250 mm×4.6 mm,5μm)色谱柱,Diamonsil预柱,流动相为乙腈-水,梯度洗脱,流速:1.0 ml·min-1,检测波长:340 nm。结果:14批苍术药材特征图谱共检出13个分离度良好的共有峰;将13个共有峰进行聚类分析,鄂西北地区苍术聚为两大类。结论:该方法简便、快捷,可作为苍术药材质量控制的有利手段。

Objective： To establish the HPLC fingerprints of Atractylodes lancea in northwest of Hubei province to provide the basis for the quality control and variety identification of Atractylodes lancea. Methods： The fingerprints were detected by HPLC. The separation was achieved on a Diamonsil^2# C18（ 250 mm × 4. 6 mm,5 μm） chromatographic column at 30℃ using acetonitrile-water（ gradient elution） as the mobile phase at a flow rate of 1. 0 ml·min^-1. The detection wavelength was 340 nm. Results： There were 13 common peaks in the HPLC fingerprints of 14 batches of Atractylodes lancea with promising separation. According to the results of clustering analysis,Atractylodes lancea in northwest of Hubei province was clustered into two categories. Conclusion： A simple,feasible and reliable method is provided for the quality control of Atractylodes lancea.