摘要
EST-SSR是基于功能基因组开发的分子标记,由于其在基因组中广泛均匀分布,具有较好的种属通用性及与表型性状相关联等优点而得到广泛应用。本研究通过下载公共数据库中蔷薇科蔷薇属,苹果属,梨属,李属,草莓属和悬钩子属的EST序列,运用生物信息学的方法,调查6个属中SSR的分布规律及特点。结果表明6个属间SSR分布频率差别较大,从悬钩子属的10.94%到梨属的43.49%。二碱基和三碱基重复类型在6个属中占据优势,其中二碱基重复类型主要为AG/CT、GA/TC及AT/TA,而三碱基重复类型在6个属中差异较大,优势类型为AGA/TCT、GAA/TTC和AAG/CTT及CAC/GTG和ACA/TGT等,显示出较好的属间特异性。该结果对于研究蔷薇科植物的分子系统发育与进化,种质资源鉴定,遗传多样性以及遗传作图提供了研究平台。
EST-SSR is a molecular marker based on functional genomics,had been widely used because of its evenly distributed among the genome,better universality between species or genera and characters associated with the phenotypic traits.We downloaded the EST sequences of six genera in Rosaceae（Rosa,Malus,Pyrus,Prunus,Fragaria and Rubus） to study the distributed characteristic of SSR in Rosaceae by bioinformatics methods.There was significant difference of EST-SSR distribution frequency among genera,from 10.94%（Rubus） to 43.49%（Pyrus）.The dinucleotide and trinucleotide repeats were the major types,AG/CT,GA/TC and AT/TA were the preponderant dinucleotide motifs,the trinucleotide repeats showed significant difference among genera,and the dominant type included AGA/TCT,GAA/TTC,AAG/CTT,CAC/GTG and ACA/TGT.Our results provided a research platform for plant molecular phylogeny and evolution,germplasm identification,genetic diversity and genetic mapping of Rosaceae.
出处
《植物研究》
CAS
CSCD
北大核心
2014年第6期810-815,共6页
Bulletin of Botanical Research
基金
国家自然基金项目(31270711)
