人羊膜材料的深低温(-196℃)保存方法可行性研究

The research of preservation methods of human amniotic material by ultra low temperature(-196℃)

目的探讨用液氮进行人羊膜深低温保存的可行性方法采用液氮深低温保存法及纯甘油4℃保存法保存人羊膜30 d、60 d和90 d,分别进行LDH活性及b FGF表达量的检测,并与新鲜人羊膜进行比较。结果用液氮深低温保存的人羊膜,在保存90 d后,其LDH活性及b FGF表达量均明显高于用纯甘油4℃保存的人羊膜,差异具有统计学意义(P<0.05),并且与新鲜羊膜LDH活性及b FGF表达量的差异不具有统计学意义(P>0.05)。结论液氮深低温保存法对羊膜进行保存的方法是安全可行的。

Objective To investigate the feasibility of the preservation method of amniotic membrane by ultra low temperature with liquid nitrogen. Methods Using the cryopreservation method of liquid nitrogen and the preservation method of pure glycerol at 4℃ to preserve human amniotic membrane for 30d, 60d and 90d, and then detected the LDH activity and bFGF expression level respectively, and compared with fresh human amniotic membrane. Result The LDH activity and the bFGF expression of the human amniotic membrane which were preserved in liquid nitrogen were significantly higher than those preserved in pure glycerine at 4 ℃, the difference was statistically significant （P 〈0.05）, and they were similar to fresh human amniotie membrane （ P〉0.05 ） . Conclusion The preservation method of the amniotic membrane with liquid nitrogen （-196℃） is safe and feasible.