LPS对人牙周膜细胞Toll样受体4及下游炎症因子表达的影响

Effect of LPS on expression of TLR4 and the downstream cytokines in PDLCs

目的:观察革兰氏阴性菌外膜成分脂多糖(LPS)对牙周膜细胞(PDLCs)Toll样受体4(TLR4)及下游炎症因子表达的影响。方法:用浓度为10μg/m L的LPS对牙周膜细胞进行刺激,Western Blot检测牙周膜细胞上TLR4受体表达,同时ELISA检测培养基上清中炎症因子TNF-α、IL-1β的含量。结果:加入LPS后,牙周膜细胞上TLR4蛋白表达增强,炎症因子TNF-α、IL-1β含量增多,且随LPS的刺激时间增加而呈上升趋势。结论:LPS促进PDLCs的TLR4表达,并导致炎症因子分泌增加,TLR4信号通路在牙周炎发病过程中具有重要作用。

Objective：To examine the effect of lipopolysaccharide（LPS） on expression of Toll-like receptor 4（TLR4） and the downstream cytokines in periodontal ligament cells （PDLCs）. Method：Expression of TLR4 in PDLCS stimulated by 10μg/mL LPS was detected by Western Blot,and the level of TNF-α,IL-1βin the supernatant were detected by ELISA. Re-sult：In PDLCs stimulated by LPS,the expression of TLR4 increased,and the level of TNF-α,IL-1β increased in a time-dependent manner. Conclusion：Stimulation with LPS could promote the expression of TLR4 and lead to increased secretion of inflammatory cytokines. TLR4 signaling pathway plays an important role in the pathogenesis of periodontitis.