摘要
目的研究人表皮生长因子样结构域蛋白-7(EGFL-7)因子在2型糖尿病大鼠脑组织中的表达以及氨基胍、Baicalein干预后对其影响。方法 48只SD大鼠随机分为正常对照组、糖尿病组、氨基胍组、Baicalein组4组,每组12只。正常对照组给予普通饮食喂养;糖尿病组、氨基胍组、Baicalein组采用高脂高糖饮食联合腹腔注射小剂量链脲佐菌素的方法建立糖尿病大鼠模型。造模成功后,氨基胍组、Baicalein组分别给予氨基胍溶液、Baicalein溶液灌胃,剂量均为150 mg/(kg·d),正常对照组、糖尿病组给予等量生理盐水灌胃。16周后,用免疫组织化学法及蛋白免疫印迹法(Western blotting)检测各组大鼠脑组织EGFL-7的表达。结果免疫组织化学法及蛋白免疫印迹法均显示,与正常对照组比较,糖尿病组、氨基胍组及Baicalein组EGFL-7的表达均明显增加(P<0.01),与糖尿病组比较,氨基胍组、Baicalein组EGFL7表达明显降低(P<0.01),Baicalein组与氨基胍组比较差异无统计学意义(P>0.05)。结论 EGFL-7可能参与糖尿病大鼠脑血管增生与重塑,氨基胍、Baicalein在一定程度上抑制其血管增生,其抑制作用可能是通过降低EGFL-7表达来实现的。
Objective To investigate the expression of epidermal growth factor-like domain 7 （EGFL-7） in brain tis？sue of diabetes mellitus （DM） rats, and the intervene effect of amioguanidine and baicalein thereof. Methods Forty-eight SD rats were randomly divided into following four groups：control group, diabetic group, aminoguanidine group and baicalein group （n=12 for each group）. The control group received only pelleted food and water. Diabetic group, aminoguanidine group and baicalein group were given high fat and sugar diet in combination with small dose of intraperitoneal injection of streptozotocin to establish the diabetic model. And then aminoguanidine group and baicalein group were given aminoguanidine and baicalein 150 mg/（kg ·d） through oral gavaging. Diabetic group and control group were given equivalent saline through oral gavaging. After 16-week treatment, the expression of EGFL-7 proteins in rat brain was detected by immunohistochemistry and Western blotting methods. Results Results of immunohistochemistry and Western blotting assay showed that the expression of EGFL-7 was significantly increased in diabetic group, aminoguanidine group and baicalein group compared with that of control group （P〈0.01）. When compared with diabetic group, the expression of EGFL-7 was significantly decreased in aminoguanidine group and baicalein group （P〈0.01）. There was no significant difference in the EGFL-7 expression between aminoguanidine group and baicalein group （P〉0.05）. Conclusion EGFL-7 may play an important role in cerebral vascular hyperplasia and remodeling of diabetic rats. Aminoguanidine and baicalein restrained cerebral vessel growth by re？ducing the expression of EGFL-7.
出处
《天津医药》
CAS
北大核心
2014年第11期1066-1068,I0001,共4页
Tianjin Medical Journal
基金
河南省科技厅科技攻关项目(122102310603)
