miRNA-204在鼻咽癌中的表达及临床意义

Clinical significance of miRNA-204 in nasopharyngeal carcinoma

目的:探讨miRNA-204在鼻咽癌(NPC)组织中的表达与临床病理特征及预后的关系。方法:收集50例NPC患者和40例鼻咽慢性炎症患者活检组织标本,采用RT-PCR方法检测miRNA-204的相对表达量,并分析其与临床病理资料和预后的相关性。结果:与鼻咽慢性炎症患者比较,miRNA-204在NPC患者血清中表达下调,其表达与NPC患者的EBV感染、分化程度、淋巴结转移和TNM分期明显相关(P<0.05),NPC患者组织中miRNA-204低表达者生存时间明显低于高表达者(P<0.05)。结论:NPC患者组织中miRNA-204的表达下调与病情进展明显相关,miRNA-204可能作为NPC治疗的一个新靶点。

Objective：To investigate the expression of miRNA-204 in patients with NPC and to analyze the association of the expression with the cIinicopathologicaI features and prognosis.Method：The expression levels of miRNA-204 in 50patients with NPC and 40 patients with nasopharyngeal chronic inflammation were detected by using stem-loop real-time reverse transcription-polymerase chain reaction RT-PCR.Analysis of the relationship between microRNA and prognosis by Kaplan-Meier method.Result：Expression levels of miRNA-204 were downregulated in patients with NPC as compared with patients with nasopharyngeal chronic inflammation（P〈0.05）.The expression of miRNA-204 was associated with EBV infection,differentiation,lymphatic metastasis and TNM stages（P〈0.05）.Low miRNA-204 expression was significantly correlated with shorter overall survival（P〈0.05）of NPC.Conclusion：The downregulation of miRNA-204 expression has related with disease progression and miRNA-204 can serve as a potential therapeutic target for NPC.