原发性胆汁性肝硬化骨髓间充质干细胞的功能研究

Characters of bone marrow mesenchymal stem cells of primary biliary cirrhosis

目的 研究PBC患者的骨髓间充质干细胞（BM-MSC）在生长与外分泌功能方面是否存在异常.方法 取PBC患者及健康对照的新鲜骨髓,体外提取并扩增BM-MSC,以统计学t检验方法比较其在生长情况和分泌细胞因子情况方面的差异.结果 ①PBC患者BM-MSC的生长情况与对照组比较差异无统计学意义;②除TGF-β1在PBC患者BM-MSC上清平均水平低于对照组外[（2.6±1.9）与（8.2±6.7） ng/ml,t=-3.641,P=0.001],PBC患者与对照组的BM-MSC在细胞因子分泌功能方面基本一致;③对照组第5代以内的BM-MSC不同代次之间在细胞因子分泌方面功能基本相同,仅在IL-10[原代与第3代（18.5±5.0）与（12.4±3.1） pg/ml,t=2.368,P=0.045]、肝细胞生长因子[原代与第2代（0.21±0.07）与（0.35±0.08） ng/ml,t=-2.874,P=0.021]水平有小范围波动.结论 PBC患者的BM-MSC在生长情况及细胞因子分泌功能方面,除TGF-β1水平略低外,与对照比较差异无统计学意义.对照组MSC第2代细胞可能更适合治疗PBC.

Objective To investigate the growth and exocrine function of BM-MSC derived from PBC patients.Methods To compare the growth patterns and cytokines secretions between PBC patients and healthy controls by student＆#39;s t test.Results ① There was no difference in growth profile and speed between PBC patients and healthy controls.② The level of TGF-β1 was much lower in the supernatant of BM-MS from OBC patients than health controls [（2.6±1.9）vs （8.2±6.7）ng/ml,t=-3.641,P=0.001].There were no other differences between two groups＆#39; BM-MSC.③ The super natant concentration of interlukin-10 of the third BM-MSC subculture from healthy controls was lower than that of the primary subculture [（18.5±5.0） vs （12.4±3.1） pg/ml,t=2.368,P=0.045],and that of hepatic growth factor from the second subcuhure was higher than the primary subculture [（0.21±0.07） vs （0.35±0.08） ng/ml,t=-2.874,P=0.021].There were seldom discrepancies in other cytokines between different generations of BM-MSC.Conclusion BM-MSC from PBC patients may have almost the similar characters in growth pattern and cytokines secretion as,except the TGF-β1,which was much lower than those from healthy controls.The second subculture of BM-MSC might be more suitable for the treatment to patients with PBC.