信号传导与转录活化因子3及其磷酸化在颅内动静脉畸形中的表达与临床相关因素分析

Correlation study of express of STAT3 and phosphorylated STAT3 in cerebral arteriovenous malformation with clinical factors

目的 明确颅内动静脉畸形（AVM）病灶内信号转导与转录活化因子3（STAT3）及其磷酸化形式的表达.方法 35例颅内AVM患者行开颅脑AVM切除术,留取手术切除标本,5例脑外伤行内减压患者脑组织为正常对照.应用免疫组化方法检测STAT3及其磷酸化形式P-STAT3（Tyr705）、P-STAT3（Ser727）的表达,并比较出血和未出血、术前栓塞和未栓塞以及Sptzer-Martin分级低级别和高级别患者STAT3及其磷酸化形式表达是否存在差异.结果 对照组中仅2例有STAT3弱表达,35例AVM患者STAT3、P-STAT3（Tyr705）和P-STAT3（Ser727）表达阳性率分别为100％、74％（26/35）和77％（27/35）.P-STAT3（Tyr705）在栓塞组表达高于未栓塞组（P＜0.05）.结论 STAT3及其磷酸化形式在颅内AVM中表达增高,STAT3通路激活可能参与颅内AVM的病理发展过程;栓塞可能促使颅内AVM病灶内STAT3活化.

Objective To investigate the expression of STAT3 （signal transducers and activators of transcription-3） and phosphorylated STAT3 in patients of cerebral arteriovenous malformation （AVM）.Methods 35 patients who underwent cerebral AVM removal had been enrolled in this study.Five patients who underwent decompression for brain trauma were normal control.The resected AVM nidus and control tissue were under immunohistochemisty analysis for STAT3,P-STAT3（Tyr705） and P-STAT3（Ser727）.Results The expression of STAT3,P-STAT3 （Tyr705） and P-STAT3 （Ser727） were 100％ （35/35）,74％ （24/35） and 77％ （24/35） respectively in AVM group.except weakly positive express of STAT3 in two patient,P-STAT3 （Tyr705） and P-STAT3 （Ser727） were all negative in control group.the expression of P-STAT3 （Tyr705） showed more intensive level in thrombosis group than unthrombosis group （P ＜ 0.05）.Conclusions STAT3 was overexpressed and activated in cerebral AVM.The activation of STAT3 signal transduction way may mediate the blood vessels remolding and neovasculization in cerebral AVM.Thrombosis maybe increase STAT3 phosphorylation.