慢性乙型病毒性肝炎脾胃湿热证与肝郁脾虚证的microRNA差异表达的初步研究

Differential Expression of MicroRNA in Chronic Hepatitis B Patients of Pi-Wei Dampness-heat Syndrome and of Gan Depression Pi Deficiency Syndrome:a Primary Research

目的探讨慢性乙型病毒性肝炎(慢性乙肝)脾胃湿热证与肝郁脾虚证之间的microRNA差异表达谱。方法运用基因芯片技术对采集的慢性乙肝脾胃湿热证组(3例)、肝郁脾虚证组(3例)患者及健康志愿者(正常组,3名)的血液标本进行microRNA检测,运用SAS系统对microRNA进行筛选及功能分析。结果脾胃湿热证组和正常组筛选出差异表达的microRNA共有77条,60条上调,17条下调;其靶基因功能主要与转录因子、气体交换、逆境刺激、酶活性调节、免疫系统的发展、肌动蛋白丝化进程等有关。肝郁脾虚证组和正常组筛选出差异表达的microRNA共41条,32条上调,9条下调;靶基因的功能主要与核苷酸或染色质的结合、转录的抑制与激活、生物的合成过程、代谢过程的调控、酶活性的调节、肌动蛋白丝化进程、调节免疫系统发展与进程、IL-12等有关。脾胃湿热证组和肝郁脾虚证组共筛选出6条差异表达的microRNA,其中上调1条,下调5条;其靶基因功能主要与跨膜转运、转录的调节、激素代谢、免疫系统的发育、肌动蛋白丝化进程、代谢过程的调节、对外部刺激的反应等有关。结论慢性乙肝脾胃湿热证与肝郁脾虚证患者血浆中存在差异表达microRNA(谱)。

Objective To explore different microRNA expression profiles between chronic hepatitis B（ CHB） patients of Pi-Wei dampness-heat syndrome（ PWDHS） and Gan depression Pi deficiency syndrome（ GDPDS）. Methods By applying gene chip technology,blood samples from CHB patients of PWDHS（ 3 cases）,GDPDS（ 3 cases）,and healthy volunteers（ 3 cases） were withdrawn and microRNA detected. The microRNA was screened and functional analyses performed by using SAS system. Results Totally 77 microRNAs with differential expression were screened from CHB patients of PWDHS and healthy volunteers,including 60 up-regulated microRNAs and 17 down-regulated microRNAs. Functions of target genes were mainly associated with transcription factors,gas exchange,adverse stimulating,regulation of enzyme activities,develo-ping of the immune system,and the process of actin filaments. Totally 41 microRNAs with differential expression were screened from CHB patients of GDPDS and healthy volunteers,including 32 up-regulated microRNAs and 9 down-regulated microRNAs. Functions of target genes were mainly associated with binding to nucleotide or chromatin,inhibition and activation of transcription,biosynthesis,regulation of metabolic process,regulation of enzyme activities,developing of the immune system,the process of actin filaments,and IL-12. Totally 6 microRNAs with differential expression were screened from CHB patients of PWDHS and CHB patients of GDPDS,including 1 up-regulated microRNA and 5 down-regulated microRNAs.Functions of target genes were mainly associated with transmembrane transport,regulation of transcription factors,metabolism of hormones,developing of the immune system,the process of actin filaments,regulation of metabolic process,response to exterior stimulation,and so on. Conclusion There existed differentially expressed microRNAs（ spectrum） between CHB patients of PWDHS and CHB patients of GDPDS.