河车虫草胶囊对慢性阻塞性肺疾病大鼠肿瘤坏死因子-α蛋白表达的影响

Effect of Heche Chongcao Capsule on Expression of TNF-α Protein in Rats with Chronic Obstructive Pulmonary Disease

目的观察慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)大鼠肺组织中肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)蛋白的表达及中药复方河车虫草胶囊对其干预的效果及机制。方法采用熏烟加气道内注入脂多糖(lipopolysaccharide,LPS)的方法制备COPD大鼠模型。将造模成功的40只SD大鼠按随机数字表法分为模型组、对照组、河车虫草胶囊低、中、高3个剂量组(简称河车低、中、高剂量组)每组8只,同时设正常组(6只),共6组。河车低、中、高剂量组分别灌胃给予河车虫草胶囊(0.25、0.5、1.0 g/kg)联合茶碱缓释片(4.5 mg/kg),对照组灌胃给予茶碱缓释片(4.5 mg/kg),1次1 m L/100 g,1天1次,共4周;同时正常组和模型组予等量蒸馏水。电镜下观察各组COPD大鼠肺组织形态学的变化;Real-time RT-PCR法检测肺组织中TNF-α蛋白表达情况。结果电镜下可见模型组支气管上皮纤毛排列紊乱,Ⅰ、Ⅱ型肺泡上皮细胞变性,内质网线粒体肿胀,板层小体排空,肺泡腔内游离碎片,各药物干预组较模型组病变程度减轻,其中河车中剂量组改善较明显。与模型组比较,各药物干预组TNF-α蛋白表达量均降低,以河车中、低剂量组更为明显(P<0.05,P<0.01);与对照组比较,河车低、中剂量组TNF-α蛋白表达量均降低(P<0.05)。结论河车虫草胶囊能有效调节COPD大鼠TNF-α蛋白的表达,抑制气道炎症反应。

Objective To observe the expression of tumor necrosis factor-alpha（ TNF-α） protein in the lung tissue of rats with chronic obstructive pulmonary disease（ COPD）,and to evaluate the intervention and mechanism of Heche Chongcao Capsule（ HCC）. Methods The COPD rat model was prepared by exposure to cigarettes smoke plus intratracheal injecting lipopolysaccharide（ LPS）. Forty successfully modeled SD rats were randomly divided into the COPD model group,the control group,the low dose HCC group,the medium dose HCC group,and the high dose HCC group,8 in each group.Meanwhile,a normal control group consisting of 6 rats was also set up. HCC at 0. 25,0. 5,and 1. 0 g / kg was administered to rats in the 3 dose HCC groups respectively by gastrogavage combined with Theophylline Sustained Release Tablet（ TSRT）.Rats in the control group were administered with TSRT by gastrogavage at 4. 5 mg / kg,1 m L /100 g each time,once daily.All medication lasted for 4 successive weeks. Equal volume of distilled water was administered by gastrogavage to rats in the COPD model group and the normal control group. Morphological changes of the lung tissue were observed under microscope.The expression of TNF-α protein in the lung tissue were also detected using Real-time PCR. Results Under electron microscope,the cilium in the tracheal epithelium was disorderly arranged,type Ⅰ and Ⅱ alveolar cells were degenerated,endo-plasmic reticulum and mitochondria were swollen,the lamellar body was emptied,and free fragment could be seen inside alveolar space. Compared with the model group,all lesions were somewhat ameliorated in all medicated groups,especially in the medium dose HCC group. Compared with the model group,the expression of TNF-α protein decreased in all medicated groups,especially in the medium and low dose HCC groups（ P 〈0. 05,P 〈0. 01）. Compared with the control group,the expression of TNF-α protein decreased in the medium and low dose HCC groups（ P 〈0. 05）. Conclusion HCC could effectively regulate the expression of TNF-α protein and inhibit airway inflammation reaction in COPD rats.