低剂量γ射线照射对人淋巴母细胞蛋白表达的影响

Effects of low dose γ-ray on protein expression of human lymphoblastoid cells

目的为探讨低剂量电离辐射生物效应作用机制，研究不同剂量单次照射后，人淋巴母细胞蛋白表达的变化。方法利用双向凝胶电泳和基质辅助激光解析飞行时间串联质谱技术分离、鉴定不同低剂量γ射线导致的人淋巴母细胞差异表达蛋白，采用实时荧光定量PCR法从mRNA水平验证蛋白的差异表达。结果不同低剂量叮射线照射后，双向电泳和质谱分离鉴定得到3个差异表达点：真核翻译起始因子5A（eIF5A），前折叠素亚单位1（PFDN1），脂肪酸结合蛋白4（FABP4）。其中，eIF5A和FABP4的mRNA水平表达变化与双向电泳结果一致。结论低剂量1射线导致人淋巴母细胞中eIF5A、PFDNl和FABP4等蛋白表达发生变化，差异表达的蛋白有助于阐明低剂量辐射生物效应作用机制，并为进一步研究低剂量辐射暴露的生物标志物提供了参考。

Objective To observe the alterations of protein expression in human normal lymphoblastoid cell AHH-1 lines, after irradiation with different doses of γ-rays, in order to investigate mechanisms of biological effects induced by low dose radiation. Methods Two-dimensional electrophoresis （2-DE） was performed and differential expression proteins were identified by matrix assisted laser desorption/ionization time of flight mass spectrometry （MAIDI-TOF-MS） after irradiation. The mRNA expressions of proteins were analyzed by real-time quantity PCR （RTQ-PCR）. Results Using 2-DE and MAIDI-TOF-MS, 3 kinds of ahered proteins were definite in terms of eukaryotic translation initiation factor 5A （eIFSA） , profoldin subunit 1 （ PFDN1 ） and fatty acid binding protein 4 （FABP4）. The mRNA expressions of eIFSA and FABP4 were coincided with protein expressions. Conclusions These differentially expressed proteins induced by low-doses of γ-rays will help to elucidate the mechanisms of biological effects at low doses and offer the reference for further research of novel biomarkers for low dose exposure.