摘要
目的探讨高表达Kiss-1基因对结肠癌SW480细胞增殖及迁移的影响。方法将p EGFP-N1-Kiss-1重组质粒转染至SW480细胞设为实验组,将p EGFP-N1质粒转染至SW480细胞设为阴性对照组,将SW480细胞设为空白对照组,采用Western blot法检测各组细胞中Kiss-1表达量;采用CCK-8法分析Kiss-1对SW480细胞增殖的影响;并用划痕实验评估Kiss-1对SW480细胞迁移能力的影响。结果将p EGFP-N1和p EGFP-N1-Kiss-1重组质粒转染至SW480结肠癌细胞,检测实验组Kiss-1表达量增加,实验组细胞增殖率明显低于对照组(P<0.05),且实验组细胞划痕损伤愈合的速度明显下降。结论高表达Kiss-1可抑制结肠癌SW480细胞的增殖,降低细胞迁移速度。
Objective To investigate the relationship between overexpression of Kiss-1 gene and the proliferation and migration of colon cancer cells.Methods The pEGFP-N1-Kiss-1 recombinant plasmid transfected into SW480 cells were desired to be experimental group,the pEGFP-N1 transfected into SW480 cells were negative control group,and normal SW480 cells were control group.Western blot was used to detect the expression of Kiss-1 in every group; CCK-8 test was used to test effect of Kiss-1 on proliferation of SW480 cells; migration was measured by wound healing assay.Results The recombinant plasmid pEGFP-N1-Kiss-1 was transfected into SW480 cells,and the expression of Kiss-1 in experimental group was significantly higher than the control group.The proliferation rate of cells in the experimental group （P ＜ 0.05） was lower than the other two groups,and the experimental group cell scratch wound healing rate dropped significantly.Conclusion Kiss-1 can inhibit the proliferation of colon cancer cells and reduce the cell migration velocity.
出处
《安徽医科大学学报》
CAS
北大核心
2014年第12期1697-1700,共4页
Acta Universitatis Medicinalis Anhui
基金
湖南省教育厅一般项目(编号:11C1107)
