结直肠腺癌中RUNX3表达与微淋巴管密度的关系及临床意义

Correlation of RUNX3 expression with microlymphatic density in colorectal adenocarcinoma tissues and its clinical significance

应用免疫组织化学方法检测30例正常结直肠组织、60例结直肠腺癌中RUNX3的表达水平,同时以D2-40为标志物,检测结直肠腺癌组织微淋巴管密度（LMVD）,分析RUNX3和LMVD与临床病例参数之间的关系,并对患者进行随访,分析两者与结直肠癌预后的关系.RUNX3蛋白在结直肠腺癌中的阳性表达率明显低于正常结直肠组织（P＜0.01）;结直肠腺癌中的LMVD值明显高于正常结直肠组织（P ＜0.01）;RUNX3表达水平和LMVD与肿瘤浸润深度、肿瘤的肝转移、淋巴结转移和TNM分期具有相关性（P＜0.01）;本组患者中51例接受了随访,中位生存期为25.5个月（7～55个月）.多因素预后分析结果显示,RUNX3表达、LMVD、癌灶浸润深度、TNM分期、肝转移及淋巴结转移是影响结直肠癌患者预后的独立危险因素。

The expressions of RUNX3 and LMVD in 30 specimens of normal colorectal tissues,and 60 specimens colorectal adenocarcinoma tissues were detected by immunohistochemistry technique.We also investigated the correlation between their expression and the clinicopathologic features.Follow up was available in selected patients.Muhivariable Cox regression was used to analyze the association between the laboratory indices and overall survival of patients with colorectal carcinoma.The positive rates of RUNX3 in colorectal adenocarcinoma tissues were significantly lower than those in normal colorectal tissues （P ＜ 0.01） ; the positive rates of LMVD in colorectal adenocarcinoma tissues were significantly higher than those in normal colorectal tissues （P ＜ 0.01）.Expression of RUNX3 and LMVD value was correlated with liver metastasis,lymph node metastasis,TNM stage and the depth of tumor invasion.In the 51 patients with followed up,the median survival was 25.5 months.Cox regression showed that RUNX3 expression,LMVD,the depth of tumor invasion,TNM stage,liver metastasis,and lymph node metastasis were independent risk factors of overall survival of colorectal carcinoma.