经皮瘤内注射188Re-锡硫胶体对兔VX2肝癌的疗效与细胞凋亡

Effect and apoptosis of percutaneous intratumoral injection with 188Re labeled stannic sulfur colloid on VX2 liver tumor in rabbits

目的 研究经皮瘤内注射88Re-锡硫胶体对兔VX2肝癌模型的疗效及细胞凋亡情况.方法 建立45只兔VX2肝癌模型,随机分为三组,每组15只.分别瘤内注射生理盐水0.1 ml（A组,对照组）、无水乙醇1 ml（B组）、37MBq 188Re-锡硫胶体0.1 ml（C组）.各组分别于术后1、4、7天处死5只动物,测量肿瘤体积,并行病理学检查及观察细胞凋亡情况.所有荷瘤兔均在术前及术后1、4、7天检测ALT、血清尿素值.结果 术后1天各组肿瘤体积差异无统计学意义（P＞0.05）;术后4天B、C组肿瘤体积[分别为（905.7±113.3）mm3和（860.2±59.6） mm3]与对照组[（1 873.1 ±77.3）mm3]比较差异有统计学意义（P＜0.01）,但B、C组间无明显差异（P =0.421）;术后7天C组[（2 169.6±141.9） mm3]与对照组[（4 093.1±126.5）mm3]比较、B组[（2 569.5±64.6）mm3]与C组比较,差异均有统计学意义（P＜0.01）.B、C组肿瘤细胞凋亡指数在各时间点均明显高于对照组,差异有统计学意义（P=0）.C组在治疗4天后凋亡指数仍处于较高水平,与B组比较差异有统计学意义（P＜0.05）.术后7天C组凋亡指数逐渐下降,与B组比较差异无统计学意义（P＞0.05）.结论 瘤内注射188Re-锡硫胶体内照射治疗VX2兔肝癌模型是一种安全有效的方法.

Objective To evaluate the efficacy and safety of rabbit VX2 liver tumor model by percutaneous intratumoral injection with 188Re labeled stannic sulfur colloid.Methods The VX2 liver tumor model was established in 45 rabbits,which were randomly assigned to 3 groups （n =15） according to material used in intratumoral injections,as follows：0.1 ml normal saline （Group A,control group）,absolute ethanol 1ml （Group B）,37MBq （1mCi） 188Re labeled stannic sulfur colloid 0.1 ml （Group C）.Five rabbits from each group were killed at intervals of 1,4 and 7d after injection and the volume of tumors were measured.Meanwhile,the histopathological changes and extent of cell apoptosis were evaluated.ALT and urea levels before the operation and at intervals of 1,4,7d post injection were also detected.Results In the first day after the injection,there was no significant statistical heterogeneity of the tumor volumes between each group.At 4th day post injection,tumor volumes of group A [（1 873.1 ± 77.3） mm3] showed significant statistical heterogeneity with group B [（905.7 ± 113.3） mm3] and C [（860.2 ± 59.6） mm3] （P ＜0.01）,while there were no obvious statistical significance between group B and group C （P =0.421）.At 7th day post injection,there were marked statistical significance of tumor volumes between A,B and C groups[respectively,（4093.1 ± 126.5）mm3,[（2569.5 ±64.6）mm3 and （2 169.6 ± 141.9）mm3]（P＜0.01）.At any time after injection,the apoptosis index （AI） of peritumoral tissue in group B and C was higher than control group with statistical significance （P ＜ 0.001）.At 4th day post injection,AI of group C remained higher than group B （P ＜ 0.05）.At 7th day,AI of group C progressively decreased,and there were no statistical difference between group B and group C （P ＞ 0.05）.Conclusion Percutaneous intratumoral injection of 188Re labeled stannic sulfur colloid is a safety and effective approach to the treatment of VX2 liver tumor in rabbits.