小桐子JcACBP基因克隆和序列分析

Cloning and Expression Analysis of a New Acyl-CoA-binding Protein (JcACBP) Identified fromJatropha curcas L.

以小桐子(Jatropha curcas)cDNA为模板,克隆了酰基辅酶A结合蛋白(Acyl-CoA-binding Protein)基因(JcACBP)的CDS序列,对其序列进行了生物信息学分析,并采用实时荧光定量PCR方法,研究了JcACBP基因在小桐子不同器官和果实生长发育阶段的表达模式。结果显示:JcACBP基因完全阅读框(coding sequence,CDS)全长279bp,编码92个氨基酸。预测其编码蛋白质的分子量为10.30kD,具有ACBP家族典型的结构域。JcACBP基因推测氨基酸与油桐(Vernicia fordii,AFZ62125)的亲缘关系最近(96%)。JcACBP基因在小桐子根、茎、叶、花、发育中的胚及果实等组织中都有表达,其中在花后40d的种子中表达最高,其次是果皮,而在根中表达较少;在果实发育过程中的表达与果实油脂积累的变化趋势基本一致。

Acyl-CoA-binding protein（ACBP）has been proposed to play apivotal role in the intracellular trafficking and the utilization of long-chain fatty acyl-CoA esters.The objective of this study is to demonstrate the structure and function of the JcACBP.A complete CDS sequence of Acyl-CoA-binding protein gene（JcACBP）was cloned from the Jatropha curcas L.We analyzed the sequence and structure characteristics of its encoded protein,and its expression patterns in the different organism and the development of fruit by quantity RT-PCR.The complete CDS sequence is 279 bp,and encoded 92 amino acid residues with aputative molecular mass of 10.30 kD.JcACBPcontained one conserved domain-ACBP superfamily.Evolutionary relationships analysis revealed a closer genetic relationship between the J.curcas L.JcACBP gene and that of Verniciafordii（96%）.JcACBPexpression significantly in different organism and had the highest transcript profile in the fruit;the expression level of JcACBPhad the same trends with the lipid accumulation in the fruit.