梾木种子低温层积过程中内源激素含量的动态变化特征

Dynamic Variation Characteristic of Endogenous Hormone Content in Cornus macrophylla Seeds during Cold Stratification

应用酶联免疫吸附测定法(ELISA)研究了梾木种子低温层积过程中内源激素含量的动态变化,分析了内源激素与种子休眠与发芽的关系。结果表明:(1)梾木种子中IAA含量在层积处理初期剧烈降低,持续一段时间后又显著升高,但后期下降,且IAA/ABA也出现同样的变化;种子中ABA含量在层积处理前期较高,但随着处理时间的延长趋于下降;种子内GA1/3含量以及GA1/3/ABA均随层积处理时间的延长逐渐增大;种子内ZRs和iPAs含量的变化相对较为平稳,尽管有一定的波动,但整体呈渐趋增高趋势。(2)梾木种子发芽率及发芽势在未经层积处理以及处理时间少于90d的条件下均为0,但随着层积处理时间的延长二者明显上升,层积处理的时间长短对梾木种子萌发有显著影响。(3)相关分析表明,梾木种子内GA1/3含量与种子的发芽率、发芽势均呈显著正相关关系,相关系数分别为0.688、0.662;种子内GA1/3/ABA增大有利于种子休眠解除和萌发。

Variations of endogenous hormone content in seeds of Cornus macrophylla during cold stratification were studied with enzyme linked immunosorbent assay（ELSA）,the relationship between endogenous hormone content and seed dormancy and germination was analyzed.The result showed that：（1）IAA content in seeds dropped in the early phase of cold stratification,but that rebounded after brief continuant time,and the variation of IAA/ABA was the same.ABA content in seeds was high before cold stratification,but as the extension of treatment time that tended to fall.Both GA1/3content and GA1/3/ABA were gradually increasing with the delay of the low-temperature stratification time.Variations of both ZRs and iPAs contents showed a smooth and steady situation,they trended to increasing despite some fluctuations.（2）The seeding experiment showed that the time of low-temperature stratification has important effects on seeds germination.Germination rate and germination potential of seeds without the low-temperature stratification treatment and these of processing time was less than 90 dwere zero,the two index rose as the extension of stratification time.Thus effect of stratification time on seed germination of C.macrophyllaseedswas obvious.（3）The results of correlation analysis between endogenous hormone and germination indexes showed that there were significantly positive correlation（P0.05）between GA1/3and germination rate,germination potential,and the correlation coefficients were 0.688 and 0.662,respectively.Increase of GA1/3/ABA could promote the releasing dormancy and germination of C.macrophyllaseeds.