大鼠心肌梗死后心功能变化与新生血管的关系

Relationship between cardiac function and angiogenesis after rat myocardial infarction

目的探讨大鼠心肌梗死后心功能变化及其与血管新生之间的关系。方法结扎大鼠左冠状动脉前降支,制备心肌梗死模型,术后利用心脏彩超分别评价梗死后1周、2周、3周和4周的心功能,采用免疫组织化学和Western blotting方法,检测各周大鼠梗死边缘Ⅷ因子和血管内皮生长因子(VEGF)的表达变化。结果大鼠心肌梗死1周时心功能最差,2周和3周心功能有所改善,4周时心功能降低;Ⅷ因子与VEGF于术后1周、2周呈高表达,3周表达开始降低,4周时已接近正常水平。结论梗死后心功能的变化与血管新生不一致,心肌梗死早期血管密度的增加不能明显改善心功能。

Objective To explore the relationship between changes of heart function and angiogenesis after myocardial infarction, and provide morphological reference for clinical treatment of myocardial infarction. Methods Acute myocardiac infarction （AMI） model was established with Sprague-Dawley rats by ligation of the left anterior descending coronary artery. Cardiac function at different weeks after AMI was evaluated by echocardiography. The change of Ⅷ factor and vascular endothelial growth factor（ VEGF） expression at the infarction edge in rats hearts at each week after AMI was detected by immunohistochemistry and Western blotting. Results The cardiac function of 1W after myocardial infarction was worst, cardiac function was improved during 2 weeks and 3weeks, but reduced at the 4th week; vm factor and VEGF in post-myocardial infarction 1 week, 2 weeks were highly expressed. Expression of both factors began to decrease at the 3rd week. At the 4th week, expression levels were close to normal. Conclusion The change of cardiac function after myocardial infarction is inconsistent with that of angiogenesis, early increased vascular density can not obviously improve cardiac function.