人参皂苷Rg1对结肠癌肿瘤体外和体内的增殖抑制作用

In vitro and in vivo inhibitory effects of ginsenoside Rg1 on proliferation of colon cancer cells

目的:研究人参皂苷Rg1在体外和体内对结肠癌的增殖抑制作用,并对其机制做初步探索.方法:体外比较正常结肠细胞与结肠癌细胞在添加不同浓度的人参皂苷Rg1以后细胞增殖情况差异,并对细胞周期及相关蛋白进行检测;对结肠癌模型小鼠灌胃给予人参皂苷Rg1,检测小鼠结肠癌生长情况,并对相关蛋白进行检测.结果:人参皂苷Rg1在体外能够特异性抑制肿瘤细胞增殖,引起细胞周期改变,在体内试验中也可以抑制肿瘤的生长,且肿瘤生长相关蛋白表达降低.结论:人参皂苷Rg1能通过下调肿瘤细胞周期及增殖相关蛋白的表达从而有效的抑制结肠癌肿瘤的增殖与生长.

AIM： To assess the inhibitory effects of ginsenoside Rg1 on colon cancer cell proliferation in vivo and in vitro, and to investigate the possible underlying mechanism. METHODS： The proliferation of normal colon cells and colon cancer cells treated with different concentrations of ginsenoside Rg1 was assessed in vitro, and related protein expression was also detected. A mouse model of colon cancer was created, and model mice were given different concentrations of ginsenoside Rg1 by gavage administration. Tumor growth and related protein expression in tumor tissue were then measured.RESULTS： Ginsenoside Rg1 could not only inhibit colon cancer cell proliferation and alter cell cycle progression in vitro, but also suppress tumor growth and decrease cancer growth related protein expression in vivo.CONCLUSION： Ginsenoside Rg1 can inhibit colon cancer growth and proliferation effectively possibly by down-regulating the expression of cell cycle- and proliferation-related proteins.