白细胞介素1和N端脑钠肽前体在糖尿病肾病诊断中的价值

Study on the Value of IL-1 and NT-pro BNP in the Diagnosis Of Diabetic Nephropathy

目的分析血中白细胞介素1(IL-1)和N端脑钠肽前体(NT-pro BNP)水平在糖尿病肾病中的诊断价值。方法选择2010年1月至2012年12月在荆州市第一人民医内分泌科住院的83例糖尿病患者为研究对象,运用荧光免疫组织化学法检测半胱氨酸蛋白酶抑制剂(Cys C)作为糖尿病肾病早期肾损伤指标将患者分为两组:糖尿病肾病组43例及非糖尿病肾病组40例,另选择15例健康体检者作为正常对照组,采用免疫组织化学发光法检测外周血中的NT-pro BNP。采用免疫比浊法检测Cys C水平,采用放射免疫法检测IL-1。结果糖尿病肾病组IL-1[(0.21±0.04)U/L vs(0.08±0.02)U/L]及NT-pro BNP[(4.73±1.02)ng/L vs(1.24±0.43)ng/L]均高于非糖尿病肾病组(P<0.05);非糖尿病肾病组IL-1及NT-pro BNP水平又高于对照组[(0.06±0.01)U/L、(0.09±0.02)ng/L],差异有统计学意义(P<0.05)。结论糖尿病肾病患者血浆IL-1及NT-pro BNP水平明显升高,在糖尿病肾病的诊断中有一定的临床价值。

Objective To analyze the correlation of serum levels of interleukin 1（ IL 1） and N-terminal pro-brain natriuretic（ NT-pro BNP） with diabetic nephropathy and its clinical diagnostic value. Methods Using Cys C level detected by fluorescence immunohistochemistry test as the indicator of early renal damage of diabetic nephropathy,the collected diabetes patients were divided into three groups at random： diabetic nephropathy group（ 43 cases）,non-diabetic nephropathy group（ 40 cases）,control group（ 15 cases of healthy people underwent physical examination）,the NT-pro BNP levels in peripheral blood of the three groups were detected by immune chemiluminescence test,and Cys C levels were detected by immune turbidimetric method,IL-1 levels were detected by radioimmunity methodt. Results In the diabetic nephropathy group and non-diabetic nephropathy group,NT-pro BNP levels were[（ 4. 73 ± 1. 02） vs（ 1. 24 ± 0. 43） ] ng / L,IL-1 levels were[（ 0. 21 ±0. 04） vs（ 0. 08 ± 0. 02） ] U / L,both levels of diabetic nephropathy group were higher than the non-diabetic nephropathy group,the differences were statistically significant（ P〈0. 05）; IL-1 and NT-pro BNP level of the non-diabetic： c nephrophethy groups were higher than the contyol group＇ s（ 0. 06 ± 0. 01） U / L and（ 0. 09 ±0. 02） ng / L respectively,the differences were statistically significant（ P〈0. 05）. Conclusion Serum levels of IL-1 and NT-pro BNP increase significantly in patients with diabetic nephropathy,therefore,detection of such levels has certain clinical value in the diagnosis of diabetic nephropathy.