响应面法建立不同经口介质中邻苯二甲酸酯生物有效性预测模型及相关因素

Response surface methodology to establish prediction models and related factors for bioaccessibility of PAEs via oral different media

运用响应面法对不同消化基质下邻苯二甲酸酯(PAEs)生物有效性的相关因素进行筛选建立预测模型,并对主要因素设定值进行分析.研究表明,3种模型中因变量与自变量之间的相关性较好(土壤:R2=0.959;农作物及植物:R2=0.973;脂肪:R2=0.862),模型拟合度较高.通过多次试验模拟,初步认为在土壤消化基质中,设置人体摄入PAEs经口摄入浓度为10μg·g-1,基质质量为0.4g,pH值约为7时,生物吸收量最高.在植物源基质下,污染物浓度在10—11μg·g-1,消化时间在6—7h,基质质量为0.4g为生物吸收量最高.脂肪源中,基质质量在0.4g,污染物浓度在10μg·g-1,脂肪量在10%—11%时,PAEs生物吸收量最高.研究通过重复试验,充分体现了该模型的准确性,及设定条件的可靠性.但本研究仅考虑摄入介质相对单一,污染物化合形态等未在考虑范围内,在后期研究中将补充试验.

Using response surface methodology for the bioaccessibility of PAEs,the relevant factors were chosen and prediction models were established under different digestion media. Studies have shown that the three models had a good correlation between the dependent and independent variables（ soil： R2= 0. 959; crops and plants： R2= 0. 973; fat： R2= 0. 862）,and model fit was high.Through several simulated experiments,it was formed that when phthalate acid esters concentration from oral intake was 10 μg·g- 1,quality was 0. 4 g,and pH was 7 in the soil media,biological absorption was highest. When phthalate acid esters concentration was between 10 μg·g- 1and 11 μg·g- 1,digestion time was from 6 h to 7 h,and quality was 0. 4 g in the crops and plants media,biological absorption was highest. When quality was 0. 4 g,phthalate acid esters concentration was10 μg·g- 1,fat was between 10% and 11%,biological absorption was highest. In the repeating tests,the model was accurate,and the condition set was reliable.