有机物对蒽酮-硫酸法测定葡萄糖含量的影响

Interference of organics on the determination of glucose by anthrone-sulfuric acid method

本文主要对污水生物处理中常见小分子有机物如乙醇、乙醛、甲酸、丙酮、乙酸异戊酯进行研究,甄别出干扰物,对不同浓度干扰物的影响及干扰物存在对不同浓度葡萄糖测定的影响进行定量分析.结果表明,乙醇对葡萄糖测定无影响,甲酸、丙酮和乙酸异戊酯使测定结果存在负偏差,乙醛使测定结果存在正偏差,且偏差值随着有机物浓度和葡萄糖浓度的增加而增大.建立了蒽酮-硫酸法葡萄糖含量测定的校正方程,研究结果可为相关领域采用该法测定葡萄糖含量提供参考.

Anthrone-sulfuric acid method is widely used to measure the glucose content. However,serious interferencesof organics always exist in the determination of glucose content in activated sludge, EPS and SMP samples when using the anthrone-sulfuric acid method. This study investigated the effects of small organics（ such as ethanol,acetaldehyde,formic acid,acetone,isoamylacetate etc.） on the glucose determination using anthrone-sulfuric acid method in biological wastewater treatment systems. The quantitative analysis was also carried out on the impacts of different concentration of interfering chemicalsonglucose determination. Results showed that the glucose determination was not affected by ethanol. There were negative deviations when formic acid,acetone and isoamyl acetate were present,while positive deviation occured when acetaldehyde was present.Furthermore,the deviation values increased with organics and glucose contents. Calibration equations for the determination of glucose content using anthrone-sulfuric acid method were established. The results can provide a reference for the determination of the glucose content in related fields.