抗结核急性药物性肝损伤患者外周血CD_8^+T细胞上CD_(28)表达及意义

CD_8^+ T cells in peripheral blood of patients with acute antituberculosis drug-induced liver injury on the expression and significance of CD_(28)

[目的]通过对抗结核急性药物性肝损伤患者外周血CD+8CD-28、CD+8CD+28细胞百分率检测,探讨其在抗结核急性药物性肝损伤中表达及意义。[方法]应用流式细胞术检测抗结核急性药物性肝损伤组42例、抗结核治疗肝功能正常组232例外周血中CD+8CD-28、CD+8CD+28细胞百分率;两组间均数的比较采用t检验,P<0.05为差异有统计学意义。[结果]抗结核急性药物性肝损伤组外周血CD+8CD-28细胞百分率低于抗结核肝功能正常组(12.2±7.7%和23.6±10.4%),两组间差异有统计学意义(t=4.782,P<0.01);抗结核急性药物性肝损伤组CD+8CD+28细胞高于抗结核肝功能正常组(23.5±9.1%和10.2±6.7%),两组间差异有统计学意义(t=5.356,P<0.01)[结论]抗结核急性药物性肝损伤外周血CD+8CD-28T细胞百分率减少,CD+8CD+28T细胞百分率增加;CD+8T细胞上CD28表达可作为抗结核急性药物性肝损伤免疫学检测标志。

Objective ] To detect the percentage of CD8＋CD2-8、CD8＋CD2＋8 cells in patients with antituberculosis drug-induced liver injury and investigate their expression on acute liver injury by antituberculosis drugs and clinical significance. [ Methods] The flow cytometry was used to detect the percentages of CD8＋CD2-8 and CD8＋CD2＋8 in peripheral blood of 42 patients with antituberculosis drug-induced acute liver injury, and 232 normal liver function patients with antituberculosis treatment.The mean amount of T cells between the two groups were compared by t-test and when P 〈0 .05 the difference found in comparison was thought to be statistically significant. [ Results] The percentage of CD8＋CD2-8 cells of antituberculosis drug-induced acute injury group was lower than that of antituberculosis with normal liver function group （12.2 ±7.7%and 23.6 ±10.4%） , and the difference was of statistical significance （ t=4.782,P〈0.01）.And the amount of CD8＋CD2＋8 cells in antituberculosis drug-induced acute injury group was higher than that in antituberculosis with normal liver function group （ 23.5 ±9.1% and 10.2 ± 6.7%）, the difference being statistically significant （t=5.356,P〈0.01） too. [Conclusion] The amount of CD8＋CD2-8 cells is reduced in peripheral blood of patients with antituberculosis drug-induced acute injury, while the amount of CD8＋CD2＋8 cells is increased.It is held that CD28 expression on CD8＋T cells can be used as a marker of immunological detection in antituberculosis drug-induced acute liver injury.