紫地榆提取物体外抗菌活性研究

The Antibacterial Activity of Extracts from Geranium strictipes R. Kunth in Vitro

目的:从紫地榆乙酸乙酯部位分离得到不同组分,并对其进行体外抗菌活性研究。方法:通过大孔树脂柱对紫地榆乙酸乙酯部位分离,分别用水和不同浓度的乙醇进行梯度洗脱,不同极性提取物用常量试管稀释法对13株常见病原菌和10株耐甲氧西林金黄色葡萄球菌进行抑菌作用研究。结果:水部位提取物对标准菌株无明显抑菌活性;50%乙醇提取物对13株常见病原菌和10株临床分离的MRSA均有不同程度的抑菌作用。结论:紫地榆50%乙醇提取物在体外有不同程度的抑菌活性。

Objective：To study the antibacterial activity in vitro of different extracts isolated from the part of ethyl acetate extracted from Geranium strictipes R. Kunth. Methods： The ethyl acetate extracts were eluted by H2O and the different concentration of the ethanol through the macro-porous resin column, then the antibacterial effects of different extracts on the 13 common pathogenic bacteria and MRSA were conducted by the method of constant tube dilution. Results： Water extract had no obviously antibacterial activity on the standard bacterial strains. 50% ethanol extracts had a certain antibacterial activity at different degree on 13 common pathogenic bacteria and 10 MRSA. Conclusion： The 50% ethanol extracts from Geranium strictipes R. Kunth have significant antibacterial effect in vitro.