新鲜全血在不同血细胞分析仪比对及偏倚评估中的应用

Application of fresh whole blood in comparison and bias estimation between different hematocyte analyzers

目的对2台不同品牌的血细胞分析仪的检测结果进行方法学比对及偏倚评估。方法以Sysmex XE-2100血细胞分析仪为参考仪器,Abbott CD-1700血细胞分析仪为比对仪器。选择新鲜全血样本40份,检测白细胞计数(WBC)、红细胞计数(RBC)、血红蛋白(HGB)、红细胞比容(HCT)、红细胞平均体积(MCV)、红细胞平均血红蛋白量(MCH)、红细胞平均血红蛋白浓度(MCHC)及血小板计数(PLT),计算相关系数(r)、线性回归方程及相对偏倚,判断2台血细胞分析仪检测结果的可比性。结果 2台血细胞分析仪的精密度好。在使用新鲜全血为校准品时,2台仪器检测的8个项目的r均大于0.975,相对偏倚均在可接受范围内。结论 2台血细胞分析仪测定结果具有可比性,用新鲜全血对不同血细胞分析仪进行比对试验,能及时发现仪器间的系统误差。

Objective To compare the detection results and estimate the bias of two hematocyte analyzers of different brands.Methods Sysmex XE-2100 hematocyte analyzer and Abbott CD-1700 hematocyte analyzer were chosen to be the reference instrument and comparison instrument,respectively.40 cases of fresh whole blood samples were collected for the detection of WBC,RBC,HGB,HCT,MCV,MCH,MCHC,and PLT respectively by the two instruments.The correlation coefficient（r）,regression equations and bias were calculated and compared to determine the comparability of the two instruments.Results The precisions of two hematocyte analyzers were satisfactory.When fresh whole blood was used as calibrators,the results of all eight items of the two instruments had good correlations（r〉0.975）,and the relative bias was acceptable.Conclusion The results of two hematocyte analyzers are comparable.With fresh whole blood using in the comparison test between different hematocyte analyzers,systematic errors can be discovered in time.